Category:Team:Groningen/Roles/Tester

From 2009.igem.org

The [http://www.upedu.org/upedu/process/workers/wk_tstr.htm Tester] role. Some of the potential activities/responsibilities include:

Researching Standard Promoter Unit, PoPS and the "Measurement kit" (see http://partsregistry.org/Measurement).
Creating (and documenting) a hierarchical collection of tests (corresponding to how the system is divided into increasingly fine-grained subsystems).
Designing (and executing) measurements for modelling/characterization of parts.
Specific tasks in the lab plan concerning measurements. In our project these could include:
- Determining where the bacteria are, what are bacterial concentrations at a certain height?
- Transporter, on/off mechanism, up to what concentration (in the cell) does it still have metal uptake.
- Measure concentration of metal. difference between begin and end concentrations of metal outside the cell.
- Expression of the gene behind the (metal sensitive) promotor (could use GFP for example).
- Measure accumulation. By measuring before/after concentration metal with and without accumulation protein.

Some examples of good measurements done previously are: [http://partsregistry.org/Part:BBa_F2620 BBa_F2620], [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/ Imperial 2007] (not sure of the exact page that was shown at the teacher's workshop), [http://partsregistry.org/Promoters/Catalog/Madras stresskit] and the table of [http://partsregistry.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection ribosome binding site strengths].

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