Duke University/10 June 2009
From 2009.igem.org
Return
June 10, 2009
- E: Gel to check PCR
- R: Smears at bottom of gel
- C: Should use Bioplastics #1 as template to increase amount of fragment 1 and Bioplastics #3 as template to increase amount of fragment
- E: PCR with Bioplastics #1 and #3 as templates and doubled volume, Gel to obtain more of fragments
- R: Smears for fragment 1, Correct bands for fragment 2, Correct bands and smears for fragment 3
- C: PCR with different conditions
- E: PCR with Bioplastics #3 as template, extension time increased to 1m 30s, cycles increased to 40