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File:Allignments fMT.jpg
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| - | Allignments of the metallothionein fMT constructs. The first is without promotor (<partinfo>BBa_K190019</partinfo>), second with constitutive promotor <partinfo>BBa_J23109</partinfo> (<partinfo>BBa_K190031</partinfo>) and the third with IPTG inducible promotor <partinfo>BBa_R0010</partinfo> (<partinfo>BBa_K190032</partinfo>). RBS is <partinfo>BBa_B0034</partinfo>. In combining the promotor and the coding sequence the Partregistry seems to add two unexpected additional bases (ag) between the two, missing in sequenced sample. In preparing the In silico construct a different prefix (lacking two bases (ag)), seen in sequenced samples, was used. The n's in the suffix of <partinfo>BBa_K190032</partinfo> were determined to be a g as expected when giving a closer look into the chromatogram. | + | Allignments of the metallothionein fMT constructs, above is In silico construct, below is sequence determined with VF2 primer. The first is without promotor (<partinfo>BBa_K190019</partinfo>), second with constitutive promotor <partinfo>BBa_J23109</partinfo> (<partinfo>BBa_K190031</partinfo>) and the third with IPTG inducible promotor <partinfo>BBa_R0010</partinfo> (<partinfo>BBa_K190032</partinfo>). RBS is <partinfo>BBa_B0034</partinfo>. In combining the promotor and the coding sequence the Partregistry seems to add two unexpected additional bases (ag) between the two, missing in sequenced sample. In preparing the In silico construct a different prefix (lacking two bases (ag)), seen in sequenced samples, was used. The n's in the suffix of <partinfo>BBa_K190032</partinfo> were determined to be a g as expected when giving a closer look into the chromatogram. |
Latest revision as of 18:48, 10 September 2009
Allignments of the metallothionein fMT constructs, above is In silico construct, below is sequence determined with VF2 primer. The first is without promotor (BBa_K190019), second with constitutive promotor BBa_J23109 (BBa_K190031) and the third with IPTG inducible promotor BBa_R0010 (BBa_K190032). RBS is BBa_B0034. In combining the promotor and the coding sequence the Partregistry seems to add two unexpected additional bases (ag) between the two, missing in sequenced sample. In preparing the In silico construct a different prefix (lacking two bases (ag)), seen in sequenced samples, was used. The n's in the suffix of BBa_K190032 were determined to be a g as expected when giving a closer look into the chromatogram.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 18:37, 10 September 2009 |  | 955×502 (157 KB) | Wilfred (Talk | contribs) | (Allignments of the metallothionein fMT constructs. The first is without promotor (BBa_K190019), second with constitutive promotor BBa_J23109 (BBa_K190031) and the third with IPTG inducible promotor BBa_R0010 (BBa_K190032). RBS is BBa_B0034. In combining t) |
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