File:Allignments fMT.jpg

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Allignments of the metallothionein fMT constructs, above is In silico construct, below is sequence determined with VF2 primer. The first is without promotor (BBa_K190019), second with constitutive promotor BBa_J23109 (BBa_K190031) and the third with IPTG inducible promotor BBa_R0010 (BBa_K190032). RBS is BBa_B0034. In combining the promotor and the coding sequence the Partregistry seems to add two unexpected additional bases (ag) between the two, missing in sequenced sample. In preparing the In silico construct a different prefix (lacking two bases (ag)), seen in sequenced samples, was used. The n's in the suffix of BBa_K190032 were determined to be a g as expected when giving a closer look into the chromatogram.

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current18:37, 10 September 2009Thumbnail for version as of 18:37, 10 September 2009955×502 (157 KB)Wilfred (Talk | contribs) (Allignments of the metallothionein fMT constructs. The first is without promotor (BBa_K190019), second with constitutive promotor BBa_J23109 (BBa_K190031) and the third with IPTG inducible promotor BBa_R0010 (BBa_K190032). RBS is BBa_B0034. In combining t)

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