Illinois/9 July 2009

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June
MTWTFSS
[http://2009.igem.org/Illinois/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
[http://2009.igem.org/Illinois/15_June_2009 15] [http://2009.igem.org/Illinois/16_June_2009 16] [http://2009.igem.org/Illinois/17_June_2009 17] [http://2009.igem.org/Illinois/18_June_2009 18] [http://2009.igem.org/Illinois/19_June_2009 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22] [http://2009.igem.org/Illinois/23_June_2009 23] [http://2009.igem.org/Illinois/24_June_2009 24] [http://2009.igem.org/Illinois/25_June_2009 25] [http://2009.igem.org/Illinois/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
[http://2009.igem.org/Illinois/29_June_2009 29] [http://2009.igem.org/Illinois/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Illinois/1_July_2009 1] [http://2009.igem.org/Illinois/2_July_2009 2] [http://2009.igem.org/Illinois/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
[http://2009.igem.org/Illinois/6_July_2009 6] [http://2009.igem.org/Illinois/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8] [http://2009.igem.org/Illinois/9_July_2009 9] [http://2009.igem.org/Illinois/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
[http://2009.igem.org/Illinois/13_July_2009 13] [http://2009.igem.org/Illinois/14_July_2009 14] [http://2009.igem.org/Illinois/15_July_2009 15] [http://2009.igem.org/Illinois/16_July_2009 16] [http://2009.igem.org/Illinois/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
[http://2009.igem.org/Illinois/20_July_2009 20] [http://2009.igem.org/Illinois/21_July_2009 21] [http://2009.igem.org/Illinois/22_July_2009 22] [http://2009.igem.org/Illinois/23_July_2009 23] [http://2009.igem.org/Illinois/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]

July 9

3'-Acting sRNAs

We cloned the GadY gene as well as the 3' UTR of gadX. We used two different primers for gadX because we weren't sure which design would work the best. Primer 2 goes all the way to the start codon of gadW.

We did this with a successful PCR.

IllinoisiGEM gadY gel1.jpg


We then purified the PCR product that was not used in the gel. We also prepared an overnight of dh5α transformed with pXG-1.


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