http://2009.igem.org/wiki/index.php?title=Judging/Variance/Newcastle&feed=atom&action=historyJudging/Variance/Newcastle - Revision history2024-03-29T05:21:53ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Judging/Variance/Newcastle&diff=71167&oldid=prevMeagan: New page: ===Team Request=== Dear iGEM judges, I would like to submit a request for variance for Team:Newcastle for the 2009 competition. As you're probably aware many teams are now synthesising f...2009-09-18T14:43:32Z<p>New page: ===Team Request=== Dear iGEM judges, I would like to submit a request for variance for Team:Newcastle for the 2009 competition. As you're probably aware many teams are now synthesising f...</p>
<p><b>New page</b></p><div>===Team Request===<br />
Dear iGEM judges,<br />
<br />
I would like to submit a request for variance for Team:Newcastle for<br />
the 2009 competition.<br />
<br />
As you're probably aware many teams are now synthesising functional<br />
components de novo to avoid lengthy cloning steps when lab time is<br />
limited. One of the iGEM sponsors is GENEART, which has an iGEM offer<br />
and supplies its synthesised parts in a number of standard vectors.<br />
<br />
GENEART supplies about 80% of constructs are in either pMA or pMK<br />
backbones. We have parts that have been supplied in pMK backbones.<br />
<br />
We understand already from the iGEM wiki that "since teams are<br />
required to submit parts in a Registry-supported plasmid backbone, you<br />
will have to transfer your GENEART-sythesized part into an acceptable<br />
plasmid backbone. Keep this in mind when planning your gene synthesis<br />
schedule."<br />
<br />
We feel however that if GENEART is going to continue to offer special<br />
offers to the competition it would facilitate the process greatly if<br />
the synthesised constructs could be submitted directly to the<br />
repository in the standard backbones that they supply. Our team is<br />
simply introducing required cloning sites to these synthesised bricks<br />
during subsequent PCR steps prior to subcloning.<br />
<br />
This would eliminate uneccessary cloning steps in the lab (especially<br />
close to the end of the competition) and speed up part deposition to<br />
the repository in order to help it grow, therefore we would ask that<br />
this variance be made.<br />
<br />
regards,<br />
<br />
Daniel Swan (on behalf of the Newcastle 2009 iGEM team)</div>Meagan