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Team Request

Dear iGEM Headquarters,

Our project this year uses mammalian microglial (BV-2) cells. As there is no current standard for mammalian plamsids, we are requesting to use pcDNA3 mammalian expression vectors. We are ordering ours from GeneScript, following their standards. They use a fairly common cloning technique, similar to iGEM. We will send our completed parts into the registry. Will you allow us to use this alternate system?

Thanks for your consideration.

Sincerely, Janie Stine and Jessamine Osborne Purdue University iGEM Team