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Team Request

Dear iGEM Headquarters,

Our project this year uses mammalian microglial (BV-2) cells. As there is no current standard for mammalian plamsids, we are requesting to use pcDNA3 mammalian expression vectors. We are ordering ours from GeneScript, following their standards. They use a fairly common cloning technique, similar to iGEM. We will send our completed parts into the registry. Will you allow us to use this alternate system?

Thanks for your consideration.

Sincerely, Janie Stine and Jessamine Osborne Purdue University iGEM Team

Judges' Response

Janie & Jessamine,

Thanks for your email.

We don't have enough information to fully consider your request.

First, do the pcDNA3 expression vectors have a bacterial replication origin (i.e., can they serve as shuttle vectors via E.coli)?

Second, is there any particular reason(s) why this vector(s) should become a standard for mammalian systems? If so, what are they?

Third, are they any mammalian vectors in the Registry (i.e., already in use) and if so what is wrong with them?

Fourth, what is the GeneScript standard?

Fifth, does the "fairly common cloning technique" that you allude to support idempotent assembly?

Sixth, is the "fairly common cloning technique" that you allude to compatible with any of the existing BioBrick physical assembly standards?

Sorry to ask so many questions, but I wanted to quickly detail the underlying issues that we need to consider.

Very happy to discuss or talk more!

All best, Drew