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Judging/Variance/UC Davis
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(New page: ===Request=== Hello - The UC Davis 2009 iGEM team would like to request a variance to allow us to submit several parts in Tom Knight's proposed fusion format (see below). We'd be happy t...)
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(New page: ===Request=== Hello - The UC Davis 2009 iGEM team would like to request a variance to allow us to submit several parts in Tom Knight's proposed fusion format (see below). We'd be happy t...)
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Revision as of 20:07, 19 August 2009
Request
Hello - The UC Davis 2009 iGEM team would like to request a variance to allow us to submit several parts in Tom Knight's proposed fusion format (see below). We'd be happy to answer any specific questions. Thank you, Marc Facciotti
Tom Knight's BBb proposal Tom Knight has recently proposed to replace BBa by the following format: Prefix Suffix 5' GAATTC...ACTAGT EcoRI SpeI ...part... GCTAGC...CTCGAG 3' NheI PstI Fusing two parts would then leave the following scar: 5' ...part A... GCTAGT A S ...part B... 3' Advantages in-frame fusion of protein parts benign protein scar N-end rule save introduction of only one new enzyme NheI is comparatively rare in the E. coli genome -- less background fragments from genomic DNA NheI can be heat-inactivated Disadvantages incompatible to BBa format incompatible to BioFusion format
