Lab Aug 29, 2009


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We need to do as many assemblies as we can today, but in between the digestion and ligation steps we need to run a gel to confirm presence of all the parts. We need to pick several (4-7) colonies from each successful plate of composite parts that was transformed on the 27th. We need to start nanospec-ing our plasmids once prepped so we can tell if DNA concentration is a limiting factor. Finally we need to deal with out p1010-kan. It looks like there should be colonies of our newly rehydrated backbone when we come in tomorrow if we are lucky.