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Lab Aug 29, 2009
From 2009.igem.org
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Jasoncummer (Talk | contribs) (New page: We need to do as many assemblies as we can today, but in between the digestion and ligation steps we need to run a gel to confirm presence of all the parts. We need to pick several (4-7) c...) |
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We need to do as many assemblies as we can today, but in between the digestion and ligation steps we need to run a gel to confirm presence of all the parts. We need to pick several (4-7) colonies from each successful plate of composite parts that was transformed on the 27th. We need to start nanospec-ing our plasmids once prepped so we can tell if DNA concentration is a limiting factor. Finally we need to deal with out p1010-kan. It looks like there should be colonies of our newly rehydrated backbone when we come in tomorrow if we are lucky. | We need to do as many assemblies as we can today, but in between the digestion and ligation steps we need to run a gel to confirm presence of all the parts. We need to pick several (4-7) colonies from each successful plate of composite parts that was transformed on the 27th. We need to start nanospec-ing our plasmids once prepped so we can tell if DNA concentration is a limiting factor. Finally we need to deal with out p1010-kan. It looks like there should be colonies of our newly rehydrated backbone when we come in tomorrow if we are lucky. | ||
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| + | Back to [https://2009.igem.org/Team:VictoriaBC/Labnotebook LAB NOTEBOOK] | ||
Latest revision as of 22:16, 17 October 2009
We need to do as many assemblies as we can today, but in between the digestion and ligation steps we need to run a gel to confirm presence of all the parts. We need to pick several (4-7) colonies from each successful plate of composite parts that was transformed on the 27th. We need to start nanospec-ing our plasmids once prepped so we can tell if DNA concentration is a limiting factor. Finally we need to deal with out p1010-kan. It looks like there should be colonies of our newly rehydrated backbone when we come in tomorrow if we are lucky.
Back to LAB NOTEBOOK
