"
Minnesota-experimental/2 July 2009
From 2009.igem.org
(Difference between revisions)
Linkri (Talk | contribs)
(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 1)'''|| width=158|'''[...)
(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 1)'''|| width=158|'''[...)
Latest revision as of 03:12, 22 October 2009
| Back to Notebook Home | |
| Go to Previous Day (July 1) | Go to Next Day (July 3) |
- Transformed TOP10 cells containing promoter constructs mutant at position 5 were screened using a fluorescent camera to select colonies producing GFP. This indicated that the cells within that colony contained plasmids with active promoters.
- Colonies which appeared green were transferred to a second antibiotic LB agar plate for further screening. The plate was marked into a numbered grid, and individual colonies were transferred into each square using a sterile micropipette tip. Cells were allowed to grow at 37 °C overnight.
