Minnesota/21 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (July 20)'''|| width=158|'''[[Minnesota/...)
 
(3 intermediate revisions not shown)
Line 5: Line 5:
|'''[[Minnesota/20 July 2009|Go to Previous Day (July 20)]]'''|| width=158|'''[[Minnesota/22 July 2009|Go to Next Day (July 22)]]'''
|'''[[Minnesota/20 July 2009|Go to Previous Day (July 20)]]'''|| width=158|'''[[Minnesota/22 July 2009|Go to Next Day (July 22)]]'''
|}
|}
 +
'''Patrick'''<br>
 +
smad_ben did not run properly. The issue that Ben was having was eliminated, so I never reran the model.<br>
 +
 +
Today I looked at how the tetR2 concentrations are behaving as a possible reason why I am finding it difficult to shift the point at which GFP concentration reaches a steady state. The issue I think is that tetR2 splits on division, and thus is diluted if tetR2 exists in appreciable quantities in the cell. This occurs at low aTc concentrations (as noted previously in the calendar). But tetR2:aTc and tetR2:aTc2 do not split on division. This is to avoid eliminating aTc from the system. So at high aTc concentrations tetR2 is much more prevalent. This problem will be explored further in the coming days.<br>
 +
 +
'''Ben'''<br>
 +
[[Image:Tnnnew16.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br>
 +
<br>
 +
[[Image:Tnnnew18.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br>

Latest revision as of 20:01, 30 July 2009

Back to Notebook Home
Go to Previous Day (July 20)Go to Next Day (July 22)

Patrick
smad_ben did not run properly. The issue that Ben was having was eliminated, so I never reran the model.

Today I looked at how the tetR2 concentrations are behaving as a possible reason why I am finding it difficult to shift the point at which GFP concentration reaches a steady state. The issue I think is that tetR2 splits on division, and thus is diluted if tetR2 exists in appreciable quantities in the cell. This occurs at low aTc concentrations (as noted previously in the calendar). But tetR2:aTc and tetR2:aTc2 do not split on division. This is to avoid eliminating aTc from the system. So at high aTc concentrations tetR2 is much more prevalent. This problem will be explored further in the coming days.

Ben
Tnnnew16.jpgTnnexperiment.jpg

Tnnnew18.jpgTnnexperiment.jpg