Minnesota/22 June 2009

From 2009.igem.org

(Difference between revisions)
(New page: {|style="align:left" width="965" |- |''' Back to Notebook Home''' |- |'''Go to Previous Day (June 19)'''|| width=158|'''[[Minnesota/...)
Line 4: Line 4:
|-
|-
|'''[[Minnesota/19 June 2009|Go to Previous Day (June 19)]]'''|| width=158|'''[[Minnesota/23 June 2009|Go to Next Day (June 23)]]'''
|'''[[Minnesota/19 June 2009|Go to Previous Day (June 19)]]'''|| width=158|'''[[Minnesota/23 June 2009|Go to Next Day (June 23)]]'''
 +
|}
 +
Here is the first incarnation of the model (many future models will simply change this one):
 +
{| class="wikitable" style="text-align:center" border = "1"
 +
|-
 +
! Reaction
 +
! Forward Kinetic Constant
 +
! Reverse Kinetic Constant
 +
|-
 +
| RNAp + lacP + lacI4:lacO1 ->  RNAp:lacP||6.23E+05||
 +
|-
 +
| RNAp + lacP + lacO1 ↔ RNAp:lacP||1E+07|| 1
 +
|-
 +
|RNA:lacP -> RNAp:lacP* || .01 ||
 +
|-
 +
|RNAp:lacP* -> lacP + lacO1 + RNAp:DNAgfp||30||
 +
|-
 +
|RNAp:DNAgfp -> RNAp + gfp_mRNA||30||
 +
|-
 +
|gfp_mRNA + rib -> rib:gfp_mRNA||100000||
 +
|-
 +
|rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA||33||
 +
|-
 +
|rib:gfp_mRNA_1 -> rib + gfp||33||
 +
|-
 +
|tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04
 +
|-
 +
|tetR2:aTc + aTc  ↔ tetR2:aTc2||1E+08||1E-03
 +
|-
 +
|tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc||1E+08||1
 +
|-
 +
|tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc2||1E+08||1E+05
 +
|-
 +
|tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc||1E+08||1E-03
 +
|-
 +
|tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc2||1E+08||1E-03
 +
|-
 +
|tetR2 -> Ø||2.89E-04||
 +
|-
 +
|tetR2:aTc -> aTc||2.89E-04||
 +
|-
 +
|tetR2:aTc2 -> 2 aTc||2.89E-04||
 +
|-
 +
|tetR2 + nsDNA ↔  tetR2:nsDNA||1000||3.2409
 +
|-
 +
|tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA||1000||3.2409
 +
|-
 +
|tetR2:aTc:nsDNA -> aTc + nsDNA||1.93E-04||
 +
|-
 +
|tetR2:nsDNA -> nsDNA||1.93E-04||
 +
|-
 +
|Ø -> tetR2||1E-11||
 +
|-
 +
|Ø -> aTc||3.3E-04||
 +
|-
 +
|gfp_mRNA -> Ø||1.16E-03||
 +
|-
 +
|gfp -> Ø||3.21E-05||
|}
|}

Revision as of 15:37, 29 July 2009

Back to Notebook Home
Go to Previous Day (June 19)Go to Next Day (June 23)

Here is the first incarnation of the model (many future models will simply change this one):

Reaction Forward Kinetic Constant Reverse Kinetic Constant
RNAp + lacP + lacI4:lacO1 -> RNAp:lacP6.23E+05
RNAp + lacP + lacO1 ↔ RNAp:lacP1E+07 1
RNA:lacP -> RNAp:lacP* .01
RNAp:lacP* -> lacP + lacO1 + RNAp:DNAgfp30
RNAp:DNAgfp -> RNAp + gfp_mRNA30
gfp_mRNA + rib -> rib:gfp_mRNA100000
rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA33
rib:gfp_mRNA_1 -> rib + gfp33
tetR2 + aTc ↔ tetR2:aTc2E+094E-04
tetR2:aTc + aTc ↔ tetR2:aTc21E+081E-03
tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc1E+081
tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc21E+081E+05
tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc1E+081E-03
tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc21E+081E-03
tetR2 -> Ø2.89E-04
tetR2:aTc -> aTc2.89E-04
tetR2:aTc2 -> 2 aTc2.89E-04
tetR2 + nsDNA ↔ tetR2:nsDNA10003.2409
tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA10003.2409
tetR2:aTc:nsDNA -> aTc + nsDNA1.93E-04
tetR2:nsDNA -> nsDNA1.93E-04
Ø -> tetR21E-11
Ø -> aTc3.3E-04
gfp_mRNA -> Ø1.16E-03
gfp -> Ø3.21E-05