Minnesota/25 June 2009

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In order to try to improve the model, the following reaction constants were changed to try to see less leakiness in the model.

Reaction Forward Kinetic Constant Reverse Kinetic Constant
RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR21200000
RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR21200000
RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR21200000


This model does indeed show some different leakiness, which is definitely a good thing, although the leakiness is still too high for an accurate model. Still, the problem remains where the model peaks and decreases instead of reaching a steady state.