Minnesota/26 June 2009

From 2009.igem.org

(Difference between revisions)
Line 21: Line 21:
[[Image:Tnnatcvariesleaky7.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br>
[[Image:Tnnatcvariesleaky7.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br>
<br>
<br>
-
The result was fairly predictable. Instead of making the model less leaky like the previous days, it increased the leakiness shown by the model. This direction is making the model worse, and so it will not be pursued in the future.
+
The result of this change was quite interesting. The curve at aTc = 0 remained constant, while the entire rest of the model has been lowered. This precisely the opposite of what is needed for the model unfortunately. This direction will probably not be pursued in the future as it is not helping solve the porblems seen in the model.

Revision as of 21:23, 30 July 2009

Back to Notebook Home
Go to Previous Day (June 25)Go to Next Day (June 29)

This model (tnn_atcvaries7_leaky) was an attempt to see what would happen if the kinetic constant was decreased for the three reactions shown below, instead of increased like the previous day's.

Reaction Forward Kinetic Constant Reverse Kinetic Constant
RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR231000
RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR231000
RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR231000


Tnnatcvariesleaky7.jpgTnnexperiment.jpg

The result of this change was quite interesting. The curve at aTc = 0 remained constant, while the entire rest of the model has been lowered. This precisely the opposite of what is needed for the model unfortunately. This direction will probably not be pursued in the future as it is not helping solve the porblems seen in the model.