Minnesota/26 June 2009
From 2009.igem.org
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This model (tnn_atcvaries7_leaky) was an attempt to see what would happen if the kinetic constant was decreased for the three reactions shown below, instead of increased like the previous day's.
Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
---|---|---|
RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2 | 31000 | |
RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2 | 31000 | |
RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2 | 31000 |
The result of this change was quite interesting. The curve at aTc = 0 remained constant, while the entire rest of the model has been lowered. This precisely the opposite of what is needed for the model unfortunately. This direction will probably not be pursued in the future as it is not helping solve the porblems seen in the model.