Minnesota/29 June 2009
From 2009.igem.org
Back to Notebook Home | |
Go to Previous Day (June 26) | Go to Next Day (June 30) |
Patrick
I switched over today to looking at making the "leakiness" equations for the TTN model. Initially I would represent the leakiness with two equations:
Rxn # | Reaction | Forward Kinetic Constant |
---|---|---|
46 | RNAp + lacP + tetO1:tetR2 + tetO2 -> RNAp:lacP | 1E+07 |
47 | RNAp + lacP + tetO1 + tetO2:tetR2 -> RNAp:lacP | 1E+07 |
The aTc complexes don't have to be represented since the leakiness is for aTc=0 in particular. Also a third leakiness equation could be made, one where both tetO1:tetR2 and tetO2:tetR2 exist, but I chose to add this later if necessary. I expect the results tomorrow.
Ben
Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
---|---|---|
RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2 | 310 | |
RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2 | 310 | |
RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2 | 310 |