# Minnesota/9 June 2009

(Difference between revisions)
 Revision as of 18:57, 29 July 2009 (view source)Patsmad (Talk | contribs)← Older edit Latest revision as of 16:26, 2 August 2009 (view source)Patsmad (Talk | contribs) (One intermediate revision not shown) Line 32: Line 32: |10||gfp -> Ø||3.21E-05|| |10||gfp -> Ø||3.21E-05|| |- |- - |11\12||tetR2 + aTc ↔ tetR2:aTc||2E+09||4E-04 + |11\12||tetR2 + aTc ↔ tetR2:aTc||1E+08||1E-03 |- |- |13\14||tetR2:aTc + aTc  ↔ tetR2:aTc2||1E+08||1E-03 |13\14||tetR2:aTc + aTc  ↔ tetR2:aTc2||1E+08||1E-03 Line 74: Line 74: |45||Ø -> aTc||3.3E-04|| |45||Ø -> aTc||3.3E-04|| |} |} +
Since we needed to have the aTc concentration constant both of the models created eliminated the aTc in all the equations and physically set each kinetic constant according to the desired aTc concentration.

## Latest revision as of 16:26, 2 August 2009

Today Patrick arrived and began working on getting up to speed on the team's TTL model and creating the TTN model he will eventually be working on.
The model created was similar to that described previously except with the addition of a second tet operon (tetO2):

Rxn # Reaction Forward Kinetic Constant Reverse Kinetic Constant
1/2RNAp + lacP + tetO1 + tetO2 ↔ RNAp:lacP1E+07 1
3RNA:lacP -> RNAp:lacP* .01
4RNAp:lacP* -> lacP + tetO1 + tetO2 + RNAp:DNAgfp30
5RNAp:DNAgfp -> RNAp + gfp_mRNA30
6gfp_mRNA + rib -> rib:gfp_mRNA100000
7rib:gfp_mRNA -> rib:gfp_mRNA_1 + gfp_mRNA33
8rib:gfp_mRNA_1 -> rib + gfp33
9gfp_mRNA -> Ø1.16E-03
10gfp -> Ø3.21E-05
11\12tetR2 + aTc ↔ tetR2:aTc1E+081E-03
13\14tetR2:aTc + aTc ↔ tetR2:aTc21E+081E-03
15\16tetR2 + tetO1 ↔ tetR2:tetO11E+081E-03
17\18tetR2:aTc + tetO1 ↔ tetR2:tetO1:aTc1E+081
19\20tetR2:aTc2 + tetO1 ↔ tetR2:tetO1:aTc21E+081E+05
21\22tetR2:tetO1 + aTc ↔ tetR2:tetO1:aTc1E+081E-03
23\24tetR2:tetO1:aTc + aTc ↔ tetR2:tetO1:aTc21E+081E-03
25\26tetR2 + tetO2 ↔ tetR2:tetO21E+081E-03
27\28tetR2:aTc + tetO2 ↔ tetR2:tetO2:aTc1E+081
29\30tetR2:aTc2 + tetO2 ↔ tetR2:tetO2:aTc21E+081E+05
31\32tetR2:tetO2 + aTc ↔ tetR2:tetO2:aTc1E+081E-03
33\34tetR2:tetO2:aTc + aTc ↔ tetR2:tetO2:aTc21E+081E-03
35tetR2 -> Ø2.89E-04
36tetR2:aTc -> aTc2.89E-04
37tetR2:aTc2 -> 2 aTc2.89E-04
38\39tetR2 + nsDNA ↔ tetR2:nsDNA10003.2409
40\41tetR2:aTc + nsDNA ↔ tetR2:aTc:nsDNA10003.2409
42tetR2:aTc:nsDNA -> aTc + nsDNA1.93E-04
43tetR2:nsDNA -> nsDNA1.93E-04
44Ø -> tetR21E-11
45Ø -> aTc3.3E-04

Since we needed to have the aTc concentration constant both of the models created eliminated the aTc in all the equations and physically set each kinetic constant according to the desired aTc concentration.