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| - | ==To find==
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| - | PDB files of LOV domain, TrpR repressor => http://www.rcsb.org/pdb/home/home.do
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| - | ==To do==
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| - | <br> <font size=3><span style="color:red"> OK </span></font size>- Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro <span style="color:red"><b> ----> will be setted up as soon as the exams are over </b></span>
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| - | <br>- Model allosteric interactions between LOVTAP & TrpR:
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| - | What will be done:
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| - | :-> model of LOVTAP in dark phase
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| - | :-> model of LOVTAP in light phase
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| - | :-> characterize how the J-alpha helix changes
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| - | We will follow the following article protocol:
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| - | <br> Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, <b> 91 </b>, 3630-3639, 2006 (javascript:PopUpMenu2_Set(Menu1630464);)
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| - | <br>- Model the fusion of LOV domain with other transcription factors
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| - | <br>- Maybe do a mutational assay to change specificity of LOVTAP:
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| - | :Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
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| - | :Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions
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| - |
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| - | ==To envisage ==
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| - | <br>- Molecular mutationnal assay
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| - | ----
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:EPF-Lausanne|Home]]
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| - | !align="center"|[[Team:EPF-Lausanne/Team|The Team]]
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| - | !align="center"|[[Team:EPF-Lausanne/Project|The Project]]
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| - | !align="center"|[[Team:EPF-Lausanne/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:EPF-Lausanne/Modeling|Modeling]]
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| - | !align="center"|[[Team:EPF-Lausanne/Notebook|Notebook]]
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| - | !align="center"|[[Team:EPF-Lausanne/Lectures|Lectures]]
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| - | !align="center"|[[Team:EPF-Lausanne/Team Management|Team Management]]
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| - | |}
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