Modelling

From 2009.igem.org

(Difference between revisions)

Revision as of 14:08, 2 June 2009

To find

PDB files of LOV domain, TrpR repressor => http://www.rcsb.org/pdb/home/home.do

To do

- Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro ----> will be setted up as soon as the exams are over
- Model allosteric interactions between LOVTAP & TrpR: What will be done:

-> model of LOVTAP in dark phase

-> model of LOVTAP in light phase

-> characterize how the J-alpha helix changes

- Model the fusion of LOV domain with other transcription factors

- Do a mutational assay to change specificity of LOVTAP:

Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)

Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions

To envisage

- Molecular mutationnal assay

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@@ Line 9: / Line 9: @@
 :-> model of LOVTAP in light phase
 :-> characterize how the J-alpha helix changes
+<br>- Model the fusion of LOV domain with other transcription factors
 <br>- Do a mutational assay to change specificity of LOVTAP:
 :Direct mutational assay: Insert mutation in specific sites thanks to the known structure of LOVTAP (already modeled on computer)
 :Indirect mutational assay: Random mutations, then selection of the "right" protein according to a set of selected conditions
-<br>- Model the fusion of LOV domain with other transcription factors
 ==To envisage ==