September/19 September 2009

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1.min prep
result of nano drop

  sample no.     conc.
     29A          18.2 ng/uL
     29B          25.7
     19A          19.2
     19B          15.7


2.cut check & ligation gel electrophoresis

19 : ligation OK


3.transformation

sample no. 27 , 28 , 30 , 35 , 48 , 50 , 51
           45 , 46
           1-1F


4.digation

K204036

VectorInsert
3-1M51-12M5
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O11dH2O11
total20uLtotal20uL


K204037

VectorInsert
3-1M51-8K5
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O11dH2O11
total20uLtotal20uL


K204038

VectorInsert
3-1M51-8I11
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O11dH2O5
total20uLtotal20uL


K204039

VectorInsert
3-1M42-8E5
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O12dH2O11
total20uLtotal20uL



37°C, O/N