http://2009.igem.org/wiki/index.php?title=Special:Contributions/Horiavulpe&feed=atom&limit=50&target=Horiavulpe&year=&month=2009.igem.org - User contributions [en]2024-03-28T09:13:17ZFrom 2009.igem.orgMediaWiki 1.16.5http://2009.igem.org/Team:McGill/ResultsTeam:McGill/Results2009-10-22T03:57:07Z<p>Horiavulpe: /* Experimental */</p>
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==Theoretical==<br />
*We have discovered through mathematical modeling the dependance on separation distance on the dynamical behaviour of activation-inhibition intercellular signaling. The results displayed in the project section of this website constitute the gathering of evidence for a proof of principle that this type of system could serve as a novel type of biological sensor.<br />
<br />
*Imagine a sensor built of bacteria grown in a general arrangement on a plate exposed to the environment. As a foreign substance (to be observed) invades the colony, the special material of the plate could lead to the distance between cells to increase/decrease (like in building fire sprinkler systems) leading to the dynamics of the cells to change, which can be empirically measured and reported. It is not necessary to have the distance between cells change, other parameters that could be used as sensors are the rate of diffusion or degradation, both of which would lead to a change in dynamics.<br />
<br />
*These are all preliminary findings. Please refer to our jamboree poster/oral presentation for more information. We also plan on continuing this project past this years iGEM competition, so feel free to contact us if you have any further questions!<br />
<br />
==Experimental==<br />
*We have observed fluorescence as predicted from cells with high copy numbers of Construct 2 (K290002), which represents the basal level of expression of EYFP from lux pL ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0063 R0063]).<br />
<br />
[[image:K290002glowsgreen.jpg|250px]]<br />
<br />
This is an indication that the K290002 construct performs as predicted, at least in so far as fluorescence is concerned.<br />
<br />
Further experiments will focus on characterizing the pRhl and lux pL promoters and, by combining the two populations of cells, on experimentally verifying the oscillations as predicted by the theoretical models.</div>Horiavulpehttp://2009.igem.org/Team:McGill/ResultsTeam:McGill/Results2009-10-22T03:52:33Z<p>Horiavulpe: /* Experimental */</p>
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==Theoretical==<br />
*We have discovered through mathematical modeling the dependance on separation distance on the dynamical behaviour of activation-inhibition intercellular signaling. The results displayed in the project section of this website constitute the gathering of evidence for a proof of principle that this type of system could serve as a novel type of biological sensor.<br />
<br />
*Imagine a sensor built of bacteria grown in a general arrangement on a plate exposed to the environment. As a foreign substance (to be observed) invades the colony, the special material of the plate could lead to the distance between cells to increase/decrease (like in building fire sprinkler systems) leading to the dynamics of the cells to change, which can be empirically measured and reported. It is not necessary to have the distance between cells change, other parameters that could be used as sensors are the rate of diffusion or degradation, both of which would lead to a change in dynamics.<br />
<br />
*These are all preliminary findings. Please refer to our jamboree poster/oral presentation for more information. We also plan on continuing this project past this years iGEM competition, so feel free to contact us if you have any further questions!<br />
<br />
==Experimental==<br />
*Ee have observed fluorescence as predicted from cells with high copy numbers of Construct 2 (K290002), which represents the basal level of expression of EYFP from lux pL ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0063 R0063]).<br />
<br />
[[image:K290002glowsgreen.jpg|250px]]<br />
<br />
This is an indication that the K290002 construct performs as predicted, at least in so far as fluorescence is concerned.<br />
<br />
Further experiments will focus on characterizing the pRhl and lux pL promoters and, by combining the two populations of cells, on experimentally verifying the oscillations as predicted by the theoretical models.</div>Horiavulpehttp://2009.igem.org/Team:McGill/ResultsTeam:McGill/Results2009-10-22T03:45:54Z<p>Horiavulpe: </p>
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==Theoretical==<br />
*We have discovered through mathematical modeling the dependance on separation distance on the dynamical behaviour of activation-inhibition intercellular signaling. The results displayed in the project section of this website constitute the gathering of evidence for a proof of principle that this type of system could serve as a novel type of biological sensor.<br />
<br />
*Imagine a sensor built of bacteria grown in a general arrangement on a plate exposed to the environment. As a foreign substance (to be observed) invades the colony, the special material of the plate could lead to the distance between cells to increase/decrease (like in building fire sprinkler systems) leading to the dynamics of the cells to change, which can be empirically measured and reported. It is not necessary to have the distance between cells change, other parameters that could be used as sensors are the rate of diffusion or degradation, both of which would lead to a change in dynamics.<br />
<br />
*These are all preliminary findings. Please refer to our jamboree poster/oral presentation for more information. We also plan on continuing this project past this years iGEM competition, so feel free to contact us if you have any further questions!<br />
<br />
==Experimental==<br />
*So far we have observed fluorescence as predicted from cells with high copy numbers of Construct 2 (K290002), which represents the basal level of expression of EYFP from lux pL ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0063 R0063]).<br />
<br />
[[image:K290002glowsgreen.jpg]]<br />
<br />
This is an indication that the K290002 construct performs as predicted, at least in so far as fluorescence is concerned.<br />
<br />
Further experiments will focus on characterizing the pRhl and lux pL promoters and, by combining the two populations of cells, on experimentally verifying the oscillations as predicted by the theoretical models.</div>Horiavulpehttp://2009.igem.org/Team:McGill/ResultsTeam:McGill/Results2009-10-22T03:40:47Z<p>Horiavulpe: </p>
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==Theoretical==<br />
*We have discovered through mathematical modeling the dependance on separation distance on the dynamical behaviour of activation-inhibition intercellular signaling. The results displayed in the project section of this website constitute the gathering of evidence for a proof of principle that this type of system could serve as a novel type of biological sensor.<br />
<br />
*Imagine a sensor built of bacteria grown in a general arrangement on a plate exposed to the environment. As a foreign substance (to be observed) invades the colony, the special material of the plate could lead to the distance between cells to increase/decrease (like in building fire sprinkler systems) leading to the dynamics of the cells to change, which can be empirically measured and reported. It is not necessary to have the distance between cells change, other parameters that could be used as sensors are the rate of diffusion or degradation, both of which would lead to a change in dynamics.<br />
<br />
*These are all preliminary findings. Please refer to our jamboree poster/oral presentation for more information. We also plan on continuing this project past this years iGEM competition, so feel free to contact us if you have any further questions!<br />
<br />
==Experimental==<br />
*So far we have observed fluorescence as predicted from cells with high copy numbers of Construct 2 (K290002), which represents the basal level of expression of EYFP from lux pL ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0063 R0063])<br />
<br />
[[image:K290002glowsgreen.jpg]]</div>Horiavulpehttp://2009.igem.org/File:K290002glowsgreen.jpgFile:K290002glowsgreen.jpg2009-10-22T03:39:53Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T03:35:44Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
==Basics==<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in <b>the presence</b> of C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
===Construct 2:===<br />
*Secretes C4-HSL in <b>the absence</b> of 3OC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002.jpg]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
===Plasmid===<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences which makes complex biobrick constructs hard to synthesize.<br />
The plasmid in which our constructs were cloned by GenScript is pCC1 from [http://www.epibio.com/main.asp Epicentre]<br />
<br />
<br />
===Cells===<br />
We use the EPI300 cells from [http://www.epibio.com/main.asp Epicentre] that were supplied by GenScript.<br />
<br />
==Planned Experiments==<br />
<br />
===Diffusion of homoserine lactones===<br />
*Both constructs can be used as receiver devices, one for 3OC6-HSL and the other for C4-HSL. If one has a lawn of cells and pipets a small amount of one of the two molecules in the centre, diffusion rates should be revealed by the rate at which cells turn on (in the case of K290003) or off (for K290002). We plan to determine the rates of diffusion of both molecules, notably for 2% Low-Melt Agarose dissolved in supplemented M9 Medium, as we plan to carry out our experiments in it.<br />
<br />
===Promoter Characterization===<br />
*In the same line of thought, by having each construct act as a receiver device, we should be able to determine the relationship between the concentration of HSL to the output from each promoter.<br />
*lux pL driven fluorescence should taper off at higher and higher concentrations of 3OC6-HSL<br />
*prhl driven fluorescence should increase at higher and higher concentrations of C4-HSL<br />
*determining the behaviour of each promoter is crucial for better predicting the oscillatory patterns we expect from the full system, which is...<br />
<br />
===Oscillator===<br />
*The system consists of a simple feedback loop between two (populations of) cells. Theoretical models predict the emergence of oscillatory behaviours in 1 dimension, where the two cells are simply split apart by a certain distance, as well as in 2 dimensions, where cells containing construct 1 are mixed with cells containing construct 2 at varying densities.<br />
[[image:OscillatorMcGill09.jpg]]</div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T03:33:58Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
==Basics==<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in ***the presence*** of C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
===Construct 2:===<br />
*Secretes C4-HSL in ***the absence*** of 3OC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002.jpg]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
===Plasmid===<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences which makes complex biobrick constructs hard to synthesize.<br />
The plasmid in which our constructs were cloned by GenScript is pCC1 from [http://www.epibio.com/main.asp Epicentre]<br />
<br />
<br />
===Cells===<br />
We use the EPI300 cells from [http://www.epibio.com/main.asp Epicentre] that were supplied by GenScript.<br />
<br />
==Planned Experiments==<br />
<br />
===Diffusion of homoserine lactones===<br />
*Both constructs can be used as receiver devices, one for 3OC6-HSL and the other for C4-HSL. If one has a lawn of cells and pipets a small amount of one of the two molecules in the centre, diffusion rates should be revealed by the rate at which cells turn on (in the case of K290003) or off (for K290002). We plan to determine the rates of diffusion of both molecules, notably for 2% Low-Melt Agarose dissolved in supplemented M9 Medium, as we plan to carry out our experiments in it.<br />
<br />
===Promoter Characterization===<br />
*In the same line of thought, by having each construct act as a receiver device, we should be able to determine the relationship between the concentration of HSL to the output from each promoter.<br />
*lux pL driven fluorescence should taper off at higher and higher concentrations of 3OC6-HSL<br />
*prhl driven fluorescence should increase at higher and higher concentrations of C4-HSL<br />
*determining the behaviour of each promoter is crucial for better predicting the oscillatory patterns we expect from the full system, which is...<br />
<br />
===Oscillator===<br />
*The system consists of a simple feedback loop between two (populations of) cells. Theoretical models predict the emergence of oscillatory behaviours in 1 dimension, where the two cells are simply split apart by a certain distance, as well as in 2 dimensions, where cells containing construct 1 are mixed with cells containing construct 2 at varying densities.<br />
[[image:OscillatorMcGill09.jpg]]</div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T03:29:29Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
==Basics==<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in response to C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
===Construct 2:===<br />
*Secretes C4-HSL in response to COC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002.jpg]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
===Plasmid===<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences which makes complex biobrick constructs hard to synthesize.<br />
The plasmid in which our constructs were cloned by GenScript is pCC1 from [http://www.epibio.com/main.asp Epicentre]<br />
<br />
<br />
===Cells===<br />
We use the EPI300 cells from [http://www.epibio.com/main.asp Epicentre] that were supplied by GenScript.<br />
<br />
==Planned Experiments==<br />
<br />
===Diffusion of homoserine lactones===<br />
*Both constructs can be used as receiver devices, one for 3OC6-HSL and the other for C4-HSL. If one has a lawn of cells and pipets a small amount of one of the two molecules in the centre, diffusion rates should be revealed by the rate at which cells turn on (in the case of K290003) or off (for K290002). We plan to determine the rates of diffusion of both molecules, notably for 2% Low-Melt Agarose dissolved in supplemented M9 Medium, as we plan to carry out our experiments in it.<br />
<br />
===Promoter Characterization===<br />
*In the same line of thought, by having each construct act as a receiver device, we should be able to determine the relationship between the concentration of HSL to the output from each promoter.<br />
*lux pL driven fluorescence should taper off at higher and higher concentrations of 3OC6-HSL<br />
*prhl driven fluorescence should increase at higher and higher concentrations of C4-HSL<br />
*determining the behaviour of each promoter is crucial for better predicting the oscillatory patterns we expect from the full system, which is...<br />
<br />
===Oscillator===<br />
*The system consists of a simple feedback loop between two (populations of) cells. Theoretical models predict the emergence of oscillatory behaviours in 1 dimension, where the two cells are simply split apart by a certain distance, as well as in 2 dimensions, where cells containing construct 1 are mixed with cells containing construct 2 at varying densities.<br />
[[image:OscillatorMcGill09.jpg]]</div>Horiavulpehttp://2009.igem.org/File:OscillatorMcGill09.jpgFile:OscillatorMcGill09.jpg2009-10-22T03:24:38Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T03:22:18Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
==Basics==<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in response to C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
===Construct 2:===<br />
*Secretes C4-HSL in response to COC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002.jpg]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
===Plasmid===<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences which makes complex biobrick constructs hard to synthesize.<br />
The plasmid in which our constructs were cloned by GenScript is pCC1 from [http://www.epibio.com/main.asp Epicentre]<br />
<br />
<br />
===Cells===<br />
We use the EPI300 cells from [http://www.epibio.com/main.asp Epicentre] that were supplied by GenScript.<br />
<br />
==Planned Experiments==<br />
<br />
===Diffusion of homoserine lactones===<br />
*Both constructs can be used as receiver devices, one for 3OC6-HSL and the other for C4-HSL. If one has a lawn of cells and pipets a small amount of one of the two molecules in the centre, diffusion rates should be revealed by the rate at which cells turn on (in the case of K290003) or off (for K290002). We plan to determine the rates of diffusion of both molecules, notably for 2% Low-Melt Agarose dissolved in supplemented M9 Medium, as we plan to carry out our experiments in it.<br />
<br />
===Promoter Characterization===<br />
*In the same line of thought, by having each construct act as a receiver device, we should be able to determine the relationship between the concentration of HSL to the output from each promoter.<br />
*lux pL driven fluorescence should taper off at higher and higher concentrations of 3OC6-HSL<br />
*prhl driven fluorescence should increase at higher and higher concentrations of C4-HSL<br />
*determining the behaviour of each promoter is crucial for better predicting the oscillatory patterns we expect from the full system, which is...<br />
<br />
===Oscillator===<br />
*<br />
[[image:OscillatorMcGill09]]</div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T03:08:28Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
===Basics===<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in response to C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
===Construct 2:===<br />
*Secretes C4-HSL in response to COC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002.jpg]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
===Plasmid===<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences which makes complex biobrick constructs hard to synthesize.<br />
The plasmid in which our constructs were cloned by GenScript is pCC1 from [http://www.epibio.com/main.asp Epicentre]<br />
<br />
<br />
===Cells===<br />
We use the EPI300 cells from [http://www.epibio.com/main.asp Epicentre] that were supplied by GenScript.<br />
<br />
==Planned Experiments==<br />
<br />
===Diffusion of homoserine lactones===<br />
*Both constructs can be used as receiver devices, one for 3OC6-HSL and the other for C4-HSL. If one has a lawn of cells and pipets a small amount of one of the two molecules in the centre, diffusion rates should be revealed by the rate at which cells turn on (in the case of K290003) or off (for K290002).<br />
We plan to determine the rates of diffusion of both molecules, especially for 2% Low-Melt Agarose dissolved in supplemented minimal medium, as we plan to carry out our experiments in such a medium.</div>Horiavulpehttp://2009.igem.org/File:K290002.jpgFile:K290002.jpg2009-10-22T02:55:37Z<p>Horiavulpe: uploaded a new version of "Image:K290002.jpg"</p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T02:54:37Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
===Basics===<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in response to C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
===Construct 2:===<br />
*Secretes C4-HSL in response to COC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002.jpg]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
Plasmid:<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences. <br />
The plasmid we use is pCC1.<br />
*LINK REGISTRY*<br />
Cells:<br />
We use EPI300 cells that were supplied by GenScript.</div>Horiavulpehttp://2009.igem.org/File:K290003.jpgFile:K290003.jpg2009-10-22T02:54:28Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/File:K290002.jpgFile:K290002.jpg2009-10-22T02:54:20Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGill/ExperimentalTeam:McGill/Experimental2009-10-22T02:51:30Z<p>Horiavulpe: </p>
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== '''Experimental Notebook''' ==<br />
<br/><br />
Experiments<br />
<br />
==Basics==<br />
<br />
Two separate cells that secrete transcription factors such that they create a feedback system between each other should interact according to the model detailed in the theory section. That is, oscillatory patterns should become apparent. In order to verify the occurrence of oscillations experimentally, we designed two gene constructs that utilize the Rhl quorum sensing system from P. aeruginosa and the Lux system from V. fischeri. <br />
<br />
===Construct 1===<br />
*Secretes 3OC6-HSL in response to C4-HSL <br />
*RhlR constitutively expressed.<br />
[[image:K290003.jpg]]<br />
<br />
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K290003 K290003]<br />
<br />
Construct 2:<br />
*Secretes C4-HSL in response to COC6-HSL<br />
*LuxR constitutively expressed.<br />
[[image:K290002]] <br />
<br />
[http://partsregistry.org/Part:BBa_K290002 K290002]<br />
<br />
Plasmid:<br />
The constructs as synthesized by GenScript were delivered in a copy-number inducible plasmid to minimize mutations. There seems to be instability due to repeats in the STOP sequences. <br />
The plasmid we use is pCC1.<br />
*LINK REGISTRY*<br />
Cells:<br />
We use EPI300 cells that were supplied by GenScript.</div>Horiavulpehttp://2009.igem.org/File:K230003.jpgFile:K230003.jpg2009-10-22T02:41:37Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGill/Notebook/HoriaTeam:McGill/Notebook/Horia2009-10-21T19:15:14Z<p>Horiavulpe: /* Horia's Notebook */</p>
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== '''Horia's Notebook''' ==<br />
<br />
Step1: make bacteria glow green.<br />
<br />
Step2: ???<br />
<br />
Step3: conquer the world.</div>Horiavulpehttp://2009.igem.org/Team:McGill/Notebook/HoriaTeam:McGill/Notebook/Horia2009-10-21T19:15:01Z<p>Horiavulpe: /* Horia's Notebook */</p>
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<br />
<br/><br />
== '''Horia's Notebook''' ==<br />
<br />
Step1: make bacteria glow green.<br />
Step2: ???<br />
Step3: conquer the world.</div>Horiavulpehttp://2009.igem.org/Team:McGill/SponsorsTeam:McGill/Sponsors2009-09-15T03:06:12Z<p>Horiavulpe: </p>
<hr />
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<h2 style="text-align:center"> We would like to thank all those who have supported us.</h2><br />
<hr /><br />
<p><br />
<table width="100%" border="1"><br />
<tr><br />
<td><div align="center"><br />
<p><a href="http://www.nce.gc.ca/comp/CECR/cecr_e.htm" target="_blank"><img src="https://static.igem.org/mediawiki/2009/0/00/Mcgill09CECR.png" width="300" height="110" /></a></p><br />
<p><b>Canadian Ministry of Industry</b></p><br />
</p><br />
</div></td><br />
<br />
<td><div align="center"><br />
<p><a href="http://www.genscript.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/b/bd/Mcgill09genscript.gif" width="300" height="100" /></a></p><br />
<p><b>&nbsp;GenScript</b></p><br />
</p><br />
</div></td><br />
<br />
</tr><br />
<tr><br />
<br />
<td><div align="center"><br />
<p><a href="http://www.nserc-crsng.gc.ca/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/4/4b/Mcgill09nserc.jpg" width="200" height="100" /></a></p><br />
<p><b>&nbsp;NSERC</b></p><br />
</p><br />
</div></td><br />
<br />
<td><div align="center"><br />
<p><a href="http://www.mcgill.ca/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/0/04/Mcgill09mcgilllogo.jpg" width="100" height="120" /></a></p><br />
<p><b>&nbsp;Mcgill University</b></p><br />
</p><br />
</div></td><br />
<br />
</tr><br />
<br />
<tr><br />
<br />
<td><div align="center"><br />
<p><a href="http://pgss.mcgill.ca/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/e/e5/Mcgill09pgss.jpg" width="300" height="100" /></a></p><br />
<p><b>&nbsp;Mcgill PGSS</b></p><br />
</p><br />
</div></td> <br />
<br />
</tr><br />
<br />
</table><br />
</html><br />
<br />
<br><br />
<br />
{| border=0 cellspacing=0 cellpadding=5 width=100% style="background:#33bbff" align=center<br />
<br />
| colspan=1 valign=top |<br />
<b><br />
Dear sponsor, <br />
<br />
By supporting McGill's 2009 iGEM competition team, you will make it possible for a group of motivated undergraduates to participate in a life changing experience. The International Genetically Engineered Machine competition is an international gathering of teams from over 100 participating schools where summer projects in synthetic biology are judged by a panel of specialists from academia and industry. Your company will be guaranteed exposure at this international even (termed, the "Jamboree") which is held at M.I.T.'s Stata Center in November 2009. The Jamboree will showcase the scientific achievements of universities and institutes from all around the world, with hundreds of students, academics, journalists as well as the general public in attendance. Our sponsors will be acknowledged during our presentation, which will be viewed by a large audience at the Jamboree, and which will also available on the internet for the general public for years to come. Your logo will also be displayed on this website, on our poster, on team T-shirts and in any conferences in which we will participate.</b><br />
<br />
<br><br><br />
Your kind donations will go towards:<br />
<br><br><br />
* the purchase of scientific equipment and supplies<br><br />
* transport to and from Boston, and accommodation<br><br />
* team t-shirts<br><br />
* registration at the competition<br><br />
<br><br />
<br />
<b>Donations to the McGill iGEM team are done via McGill University, and hence are considered a charitable gift. McGill's Business Number is 11912 8981 RR0001</b><br />
<br />
|-<br />
|}<br />
<br />
<br><p><br />
[[Image:jamboree1.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] [[Image:mcgilljam_3.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] [[Image:jamboree4.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] [[Image:jamboree3.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] <br />
<br />
<br><br clear="all"><br />
<br />
<br />
==Get in Touch==<br />
<br />
* <b>McGill iGEM Team</b><br><br />
* <b>Email: horia.vulpe@mail.mcgill.ca</b><br />
* <b>Email: fnaqib@gmail.com</b><br />
<br />
c/o Dr. Jay Louise Nadeau<br><br />
3775 University Street, Room 310<br><br />
Montreal, PQ. H3A 2B4<br><br />
1(514) 398-8372 <br><br />
jay.nadeau@mcgill.ca <br><br />
<br></div>Horiavulpehttp://2009.igem.org/Team:McGill/TeamTeam:McGill/Team2009-09-15T02:59:54Z<p>Horiavulpe: </p>
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<br />
=='''Who we are'''==<br />
<br />
'''Advisors:'''<br />
{|border=1 width=40% cellpadding="0" cellspacing="0"<br />
|-<br />
| [[Image : Mcgill09Leon.jpg|thumb|center|150px|[http://www.cnd.mcgill.ca/bios/glass/glass.html Dr. Leon Glass]]]<br />
| [[Image : Mcgill09Jay.jpg |thumb|center|150px|[http://www.mcgill.ca/microimm/department/associate_adjunct_prof/nadeau/ Dr. Jay Nadeau]]]<br />
|}<br />
<br/><br />
<br />
'''Students:'''<br />
{|border=1 width=80% cellpadding="0" cellspacing="0"<br />
|-<br />
| [[Image:Mcgill09Faisal2.jpg|thumb|center|200px| [[Team:McGill/Faisal|Faisal Naqib]] ]]<br />
| [[Image:horiavulpe2.jpg|thumb|center|200px| [[Team:McGill/Horia|Horia Vulpe]] ]]<br />
| [[Image:Thomasquail1.jpg|thumb|center|200px| Thomas Quail]]<br />
| [[Image:Louaimusa1.jpg|thumb|center|200px| Louai Musa]]<br />
|}<br />
<br/><br />
<br />
== '''The Team''' ==<br />
[[Image:Mcgill09Team.jpg|border|500px|center|The Team:(Left to Right)Tom,Louai,Faisal,Horia]]<br />
<br />
<br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)</div>Horiavulpehttp://2009.igem.org/File:Louaimusa1.jpgFile:Louaimusa1.jpg2009-09-15T02:57:12Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/File:Thomasquail1.jpgFile:Thomasquail1.jpg2009-09-15T02:57:07Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGillTeam:McGill2009-09-15T02:50:26Z<p>Horiavulpe: </p>
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</html></div>Horiavulpehttp://2009.igem.org/Team:McGill/SponsorsTeam:McGill/Sponsors2009-09-15T02:30:50Z<p>Horiavulpe: </p>
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<h2 style="text-align:center"> We would like to thank all those who have supported us.</h2><br />
<hr /><br />
<p><br />
<table width="100%" border="1"><br />
<tr><br />
<td><div align="center"><br />
<p><a href="http://www.nce.gc.ca/comp/CECR/cecr_e.htm" target="_blank"><img src="https://static.igem.org/mediawiki/2009/0/00/Mcgill09CECR.png" width="300" height="110" /></a></p><br />
<p><b>Canadian Ministry of Industry</b></p><br />
</p><br />
</div></td><br />
<br />
<td><div align="center"><br />
<p><a href="http://www.genscript.com/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/b/bd/Mcgill09genscript.gif" width="300" height="100" /></a></p><br />
<p><b>&nbsp;GenScript</b></p><br />
</p><br />
</div></td><br />
<br />
</tr><br />
<tr><br />
<br />
<td><div align="center"><br />
<p><a href="http://www.nserc-crsng.gc.ca/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/4/4b/Mcgill09nserc.jpg" width="200" height="100" /></a></p><br />
<p><b>&nbsp;NSERC</b></p><br />
</p><br />
</div></td><br />
<br />
<td><div align="center"><br />
<p><a href="http://www.mcgill.ca/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/0/04/Mcgill09mcgilllogo.jpg" width="100" height="120" /></a></p><br />
<p><b>&nbsp;Mcgill University</b></p><br />
</p><br />
</div></td><br />
<br />
</tr><br />
<br />
<tr><br />
<br />
<td><div align="center"><br />
<p><a href="http://pgss.mcgill.ca/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/e/e5/Mcgill09pgss.jpg" width="300" height="100" /></a></p><br />
<p><b>&nbsp;Mcgill PGSS</b></p><br />
</p><br />
</div></td> <br />
<br />
</tr><br />
<br />
</table><br />
</html><br />
{|style="font color="#ffffff"; background-color:#cd0000; cellpadding="3" cellspacing="5" border="2" bordercolor="#cd0000"border-spacing:6px; text-align:center" width="960px"<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal|<font color="#ffffff">Home</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Team|<font color="#ffffff">The Team</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Project|<font color="#ffffff">The Project</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Parts|<font color="#ffffff">Parts</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Notebook|<font color="#ffffff">Notebook</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Modeling|<font color="#ffffff">Modeling</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Links|<font color="#ffffff">Links</font>]]<br />
!style="text-align:center; background-color:#cd0000; border-width:0px; padding:3px;"|[[Team:Montreal/Sponsors|<font color="#ffffff">Sponsors</font>]]<br />
|}<br />
<br><br />
<br />
{| border=0 cellspacing=0 cellpadding=5 width=100% style="background:#33bbff" align=center<br />
<br />
| colspan=1 valign=top |Dear sponsor, <br />
<br />
By supporting McGill's 2009 iGEM competition team, you will make it possible for a group of motivated undergraduates to participate in a life changing experience. The International Genetically Engineered Machine competition is an international gathering of teams from over 100 participating schools where summer projects in synthetic biology are judged by a panel of specialists from academia and industry. Your company will be guaranteed exposure at this international even (termed, the "Jamboree") which is held at M.I.T.'s Stata Center in November 2009. The Jamboree will showcase the scientific achievements of universities and institutes from all around the world, with hundreds of students, academics, journalists as well as the general public in attendance. Our sponsors will be acknowledged during our presentation, which will be viewed by a large audience at the Jamboree, and which will also available on the internet for the general public for years to come. Your logo will also be displayed on this website, on our poster, on team T-shirts and in any conferences in which we will participate.<br />
<br />
<br><br><br />
Your kind donations will go towards:<br />
<br><br><br />
* the purchase of scientific equipment and supplies<br><br />
* transport to and from Boston, and accommodation<br><br />
* team t-shirts<br><br />
* registration at the competition<br><br />
<br><br />
<br />
<b>Donations to the McGill iGEM team are done via McGill University, and hence are considered a charitable gift. McGill's Business Number is 11912 8981 RR0001</b><br />
<br />
|-<br />
|}<br />
<br />
<br><p><br />
[[Image:jamboree1.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] [[Image:mcgilljam_3.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] [[Image:jamboree4.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] [[Image:jamboree3.jpg|200px|left|photos: Mackenzie L. Cowell (Creative Commons)]] <br />
<br />
<br><br clear="all"><br />
<br />
<br />
==Get in Touch==<br />
<br />
* <b>McGill iGEM Team</b><br><br />
* <b>Email: horia.vulpe@mail.mcgill.ca</b><br />
* <b>Email: fnaqib@gmail.com</b><br />
<br />
c/o Dr. Jay Louise Nadeau<br><br />
3775 University Street, Room 310<br><br />
Montreal, PQ. H3A 2B4<br><br />
1(514) 398-8372 <br><br />
jay.nadeau@mcgill.ca <br><br />
<br></div>Horiavulpehttp://2009.igem.org/File:Jamboree3.jpgFile:Jamboree3.jpg2009-09-15T02:29:00Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/File:Jamboree4.jpgFile:Jamboree4.jpg2009-09-15T02:28:53Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/File:Mcgilljam_3.jpgFile:Mcgilljam 3.jpg2009-09-15T02:27:46Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/File:Jamboree1.jpgFile:Jamboree1.jpg2009-09-15T02:27:21Z<p>Horiavulpe: </p>
<hr />
<div></div>Horiavulpehttp://2009.igem.org/Team:McGill/ResultsTeam:McGill/Results2009-09-15T02:20:24Z<p>Horiavulpe: </p>
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*Coming Soon!</div>Horiavulpehttp://2009.igem.org/Team:McGill/TeamTeam:McGill/Team2009-08-24T23:24:54Z<p>Horiavulpe: /* Who we are */</p>
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=='''Who we are'''==<br />
<br />
'''Advisors:'''<br />
{|border=1 width=40% cellpadding="0" cellspacing="0"<br />
|-<br />
| [[Image : Mcgill09Leon.jpg|thumb|center|150px|[http://www.cnd.mcgill.ca/bios/glass/glass.html Dr. Leon Glass]]]<br />
| [[Image : Mcgill09Jay.jpg |thumb|center|150px|[http://www.mcgill.ca/microimm/department/associate_adjunct_prof/nadeau/ Dr. Jay Nadeau]]]<br />
|}<br />
<br/><br />
<br />
'''Students:'''<br />
{|border=1 width=80% cellpadding="0" cellspacing="0"<br />
|-<br />
| [[Image:Mcgill09Faisal2.jpg|thumb|center|150px| [[Team:McGill/Faisal|Faisal Naqib]] ]]<br />
| [[Image:horiavulpe2.jpg|thumb|center|150px| [[Team:McGill/Horia|Horia Vulpe]] ]]<br />
| [[Image:Mcgill09Faisal3.jpg|thumb|center|150px| Thomas Quail]]<br />
| [[Image:Mcgill09Faisal4.jpg|thumb|center|150px| Louai Musa]]<br />
|}<br />
<br/><br />
<br />
== '''The Team''' ==<br />
[[Image:Mcgill09Team.jpg|border|500px|center|The Team:(Left to Right)Tom,Louai,Faisal,Horia]]<br />
<br />
<br />
<br />
== '''What we did''' ==<br />
<br />
(Provide proper attribution for all work)</div>Horiavulpehttp://2009.igem.org/File:Horiavulpe2.jpgFile:Horiavulpe2.jpg2009-08-24T23:21:15Z<p>Horiavulpe: </p>
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<div></div>Horiavulpe