Team:Alberta/Project/AnchTermAnneal

From 2009.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 39: Line 39:
To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp.
To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp.
 +
 +
 +
<ul>
 +
<li>Combine 10 μL of Anchor/Term_(A or B) and Anchor/Term_Comp 100 μM stock solutions into an eppendorf tube.
 +
<li>Add 80 μL water or TE, mix.
 +
<li>Heat solution to 95C for 2 minutes.
 +
<li>Let cool to room temperature slowly.
 +
</ul>

Latest revision as of 16:52, 21 October 2009

University of Alberta - BioBytes










































































































Annealing Anchor and Terminator Bytes

Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10μM).

What you will need:

  • Anchor oligos (complementary)
  • Terminator oligos (complementary)

Procedure:

To make a 10 μM Anchor solution with an A(B) overhang one must anneal Anchor_A(Anchor_B) and Anchor_Comp. To make a 10 μM Terminator solution with an A(B) overhang one must anneal Term_A(Term_B) and Term_Comp.
  • Combine 10 μL of Anchor/Term_(A or B) and Anchor/Term_Comp 100 μM stock solutions into an eppendorf tube.
  • Add 80 μL water or TE, mix.
  • Heat solution to 95C for 2 minutes.
  • Let cool to room temperature slowly.

Retrieved from "http://2009.igem.org/Team:Alberta/Project/AnchTermAnneal"