"
Team:Alberta/Project/Recombineering
From 2009.igem.org
(Difference between revisions)
| Line 52: | Line 52: | ||
<td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"> | <td style="height: 400; padding-left: 10px; padding-right: 10px; padding-top: 11px;"> | ||
<b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b> | <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b> | ||
| - | <div class=" | + | <div class="What is recombineering?"> |
<div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"> | <div style="height: 400; background:#FFFFFF; colorou line-height:100% padding: 3px 0px;"> | ||
| - | <h1> | + | <h1>Targeting<h1> |
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --> | <!-- <div align="justify" style="padding-left:20px; padding-right:20px"> --> | ||
Revision as of 20:54, 17 September 2009
 |
|
|
What is Recombineering?Recombineering refers to the strategic use of recombination in vivo in order to reach a defined goal. In the case of BioBytes, a method is required to target the final construct to insertion at a specific place on the E. coli chromosome. To do this successfully, three components must be taken into account: - There must be a system for targeting the construct to a specific site for insertion - Activation of the recombination system must be under experimenter control - It must be possible to select for and verify colonies in which the insertion was successful |
Targeting |
