Team:ArtScienceBangalore/Notebook/Week Four

From 2009.igem.org

(Difference between revisions)
(→June 9th)
(→June 8th- 17th)
Line 36: Line 36:
How we tried to do this:
How we tried to do this:
-
'''[[Step 1 - Taking dry DNA from wells]]'''
+
'''[http://hackteria.org/wiki/index.php/Step_1_-_Taking_dry_DNA_from_wells Step 1 - Taking dry DNA from wells]'''
-
'''[[Step 2 - Transforming competent cells]]'''
+
'''[http://hackteria.org/wiki/index.php/Step_2_-_Transforming_competent_cells Step 2 - Transforming competent cells]'''
'''Step 3 - Picking a single colony.'''
'''Step 3 - Picking a single colony.'''
Line 44: Line 44:
'''Step 4 - Inoculating broth with Ampicillin-R''' (an antibiotic) and letting it grow for 18 hours.
'''Step 4 - Inoculating broth with Ampicillin-R''' (an antibiotic) and letting it grow for 18 hours.
-
'''Step 5 - Using the resulting culture for [[mini-prep.]]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
+
'''Step 5 - Using the resulting culture for [http://hackteria.org/wiki/index.php/Mini-prep. mini-prep.]''' - ''a process used to purify plasmids and yields clean, usable DNA.''
-
'''Step 6 - [[Digesting the DNA]]'''
+
'''Step 6 - [http://hackteria.org/wiki/index.php/Digesting_the_DNA Digesting the DNA]'''
-
'''Step 7 - [[Gel Electrophoresis]]'''
+
'''Step 7 - [http://hackteria.org/wiki/index.php/Gel_Electrophoresis Gel Electrophoresis]'''
-
'''Step 8-  [[Ligation]]'''
+
'''Step 8-  [http://hackteria.org/wiki/index.php/Ligation Ligation]'''
'''Step 9-  [[Transformation and Inoculation]]'''
'''Step 9-  [[Transformation and Inoculation]]'''

Revision as of 19:40, 21 October 2009

Week Four

June 8th- 17th

What are we trying to do?

We want the bacteria to lyse, to kill itself from the inside.

Well then, to do that we had to get a lysis gene in it. The thing is, with the lysis gene needs to be turned on by this specific substance called a promoter

There are two kinds of promoters, ones that make the gene they're attached to , be 'on' all the time. Then, you have ones that turn on when you want them to, in our case, when you add a mystery enzyme.

Our Step-wise Process:

How we tried to do this:

Step 1 - Taking dry DNA from wells

Step 2 - Transforming competent cells

Step 3 - Picking a single colony.

Step 4 - Inoculating broth with Ampicillin-R (an antibiotic) and letting it grow for 18 hours.

Step 5 - Using the resulting culture for mini-prep. - a process used to purify plasmids and yields clean, usable DNA.

Step 6 - Digesting the DNA

Step 7 - Gel Electrophoresis

Step 8- Ligation

Step 9- Transformation and Inoculation

June 9th

The June 9th Image Gallery

Images of the various results attained:

Agarose gel electrophoresis image of digested K112808

Failure 1

The pBAD Insert

The Failed Ligation Attempt

Retrieved from "http://2009.igem.org/Team:ArtScienceBangalore/Notebook/Week_Four"