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Team:BIOTEC Dresden/Notebook v2
From 2009.igem.org
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| + | === Sub-Projects === | ||
'''[[Team:BIOTEC_Dresden/Notebook_Recombinase|FLP Recombinase-based PoPS Measurement Device ]]''' | '''[[Team:BIOTEC_Dresden/Notebook_Recombinase|FLP Recombinase-based PoPS Measurement Device ]]''' | ||
| - | Utilizing a new biobrick, | + | Utilizing a new biobrick, the FLP reporter system can used to determine the transcription rate of gene expression. It can be applied to measure the persistence length of DNA by using different spacings between the FLP recombinase sites. |
| + | '''[[Team:BIOTEC_Dresden/Notebook_Vesicles|Gene Expression in Vesicles ]]''' | ||
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| + | Instead of gene expression in cells, it is attempted to express this system in vitro, using lipid vesicles. First of all, a method to create those vesicles is introduced, then a gene expression kit is inserted. | ||
'''[[Team:BIOTEC_Dresden/Notebook_SilverNano|Silver Nano-Particles ]]''' | '''[[Team:BIOTEC_Dresden/Notebook_SilverNano|Silver Nano-Particles ]]''' | ||
| - | + | Following the description given in ((add reference)), it is attempted to create nanoparticles using a silver-binding peptide. This is a promising approach for tagging proteins, but it turns out that the protocol is insufficient. | |
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| + | === Calendar === | ||
The [http://www.google.com/calendar/embed?height=600&wkst=1&bgcolor=%23FFFFFF&src=igem.biotec%40googlemail.com&color=%23AB8B00&ctz=Europe%2FBerlin/ Team calender] is available here | The [http://www.google.com/calendar/embed?height=600&wkst=1&bgcolor=%23FFFFFF&src=igem.biotec%40googlemail.com&color=%23AB8B00&ctz=Europe%2FBerlin/ Team calender] is available here | ||
Revision as of 09:46, 20 October 2009
Sub-Projects
FLP Recombinase-based PoPS Measurement Device
Utilizing a new biobrick, the FLP reporter system can used to determine the transcription rate of gene expression. It can be applied to measure the persistence length of DNA by using different spacings between the FLP recombinase sites.
Instead of gene expression in cells, it is attempted to express this system in vitro, using lipid vesicles. First of all, a method to create those vesicles is introduced, then a gene expression kit is inserted.
Following the description given in ((add reference)), it is attempted to create nanoparticles using a silver-binding peptide. This is a promising approach for tagging proteins, but it turns out that the protocol is insufficient.
Calendar
The [http://www.google.com/calendar/embed?height=600&wkst=1&bgcolor=%23FFFFFF&src=igem.biotec%40googlemail.com&color=%23AB8B00&ctz=Europe%2FBerlin/ Team calender] is available here
