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Team:BIOTEC Dresden/Results SilverNano
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=== Silver Nano Particles - Results === | === Silver Nano Particles - Results === | ||
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=====Spectrophotometry===== | =====Spectrophotometry===== | ||
| - | + | Figure 1 shows the observed absorption spectrum for silver nanoparticles reported by Naik in doi:10.1038/nmat758. Figure 2 shows the spectrum observed from the incubated sample containing silver binding peptide, silver nitrate and sodium ascorbate. That the production of silver nanoparticles is explicitly due to the presence of the peptide AG4 is confirmed by the absence of expected the absorbance peaks (400-500nm) in a negative control containing only buffers, silver nitrate, and sodium ascorbate. | |
| - | A good agreement is observed, the deviation can be explained as a result of a different size distribution in our sample. | + | A good agreement is observed, the deviation can be explained as a result of a different size distribution in our sample as further confirmed by SEM. |
[[Image:Silver4.jpg|350px|left|Fig.1 expected UV/Vis spectra of silver nanoparticle on Ag4]] | [[Image:Silver4.jpg|350px|left|Fig.1 expected UV/Vis spectra of silver nanoparticle on Ag4]] | ||
[[Image:Silver5.jpg|350px|right|Fig.2 UV/Vis spectra of incubated silver binding peptide]] | [[Image:Silver5.jpg|350px|right|Fig.2 UV/Vis spectra of incubated silver binding peptide]] | ||
| + | =====Electron Microscopy===== | ||
| + | Electron micrographs of an incubated sample of silver binding peptide, with Sodium Ascorbate, Silver Nitrate, and water. Crystalline structures indicating the presence metallic particles are clearly visible. | ||
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| + | {| | ||
| + | | [[Image:Silver2.jpg|thumb|1000|alt=electron microscopy on silver nanoparticles|]] | ||
| + | | [[Image:Silver3.jpg|thumb|1000|alt=electron microscopy on silver nanoparticles|]] | ||
| + | |} | ||
The protocol to create silver particles works and can be combined with the gene expression in vesicles to influence the size distribution. | The protocol to create silver particles works and can be combined with the gene expression in vesicles to influence the size distribution. | ||
{{:Team:BIOTEC_Dresden/NewTemplateEnd}} | {{:Team:BIOTEC_Dresden/NewTemplateEnd}} | ||
Revision as of 03:53, 22 October 2009
Silver Nano Particles - Results
Spectrophotometry
Figure 1 shows the observed absorption spectrum for silver nanoparticles reported by Naik in doi:10.1038/nmat758. Figure 2 shows the spectrum observed from the incubated sample containing silver binding peptide, silver nitrate and sodium ascorbate. That the production of silver nanoparticles is explicitly due to the presence of the peptide AG4 is confirmed by the absence of expected the absorbance peaks (400-500nm) in a negative control containing only buffers, silver nitrate, and sodium ascorbate.
A good agreement is observed, the deviation can be explained as a result of a different size distribution in our sample as further confirmed by SEM.
Electron Microscopy
Electron micrographs of an incubated sample of silver binding peptide, with Sodium Ascorbate, Silver Nitrate, and water. Crystalline structures indicating the presence metallic particles are clearly visible.
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The protocol to create silver particles works and can be combined with the gene expression in vesicles to influence the size distribution.
