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Team:Berkeley Wetlab/Passenger: Streptavidin
From 2009.igem.org
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With the display of a numerous number of proteins on the surface, there is a need determine the viability of the proteins. One such method is to tag the displayed proteins. By displaying the strepavidin binding protein, we can effectively tag displayers as well as other proteins of interest. The strepavidin binding protein is a short peptide sequence SAECHPQGPPCIEGRK that bind onto strepavidin. Using a streptavidin, R-phycoerythrin conjugate (SAPE), we can fluorescently tag proteins and displayers for high through-put, automated analysis. | With the display of a numerous number of proteins on the surface, there is a need determine the viability of the proteins. One such method is to tag the displayed proteins. By displaying the strepavidin binding protein, we can effectively tag displayers as well as other proteins of interest. The strepavidin binding protein is a short peptide sequence SAECHPQGPPCIEGRK that bind onto strepavidin. Using a streptavidin, R-phycoerythrin conjugate (SAPE), we can fluorescently tag proteins and displayers for high through-put, automated analysis. | ||
| - | ==Functional Assay: | + | ==Functional Assay: Fluorescent Plate Reader== |
Revision as of 22:15, 20 October 2009
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Strepavidin Binding Peptide
With the display of a numerous number of proteins on the surface, there is a need determine the viability of the proteins. One such method is to tag the displayed proteins. By displaying the strepavidin binding protein, we can effectively tag displayers as well as other proteins of interest. The strepavidin binding protein is a short peptide sequence SAECHPQGPPCIEGRK that bind onto strepavidin. Using a streptavidin, R-phycoerythrin conjugate (SAPE), we can fluorescently tag proteins and displayers for high through-put, automated analysis.
