Team:Bologna

From 2009.igem.org

(Difference between revisions)
(Project Summary)
(Project Summary)
Line 26: Line 26:
<html>
<html>
<font face="Calibri" font size="4" color="#000000">
<font face="Calibri" font size="4" color="#000000">
-
The device consists of two new BioBricks:
+
The device consists of two new BioBricks (see Fig.1):
<br><br>
<br><br>
<ul>
<ul>
Line 38: Line 38:
CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.
CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.
<br><br>
<br><br>
-
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The <b>RNA duplex</b> prevents ribosome from binding to RBS, <b>repressing protein synthesis</b>. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate. </font>
 
</html>
</html>
<br><br><br>
<br><br><br>
[[Image:project3b.png|center|950px|]]
[[Image:project3b.png|center|950px|]]
 +
Fig.1
<br><br>
<br><br>
-
 
+
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The <b>RNA duplex</b> prevents ribosome from binding to RBS, <b>repressing protein synthesis</b>. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate. </font>
<font face="Calibri" size="5">
<font face="Calibri" size="5">
'''How can we test the device?'''
'''How can we test the device?'''

Revision as of 16:36, 21 October 2009

ProvaBol2.png
HOME TEAM PROJECT SOFTWARE MODELING WET LAB PARTS HUMAN PRACTICE JUDGING CRITERIA




Ely9Copia.jpg


Project Summary


Which is our idea?

The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device T-Rex (Trans Repressor of Expression).


How does T-Rex work?

The device consists of two new BioBricks (see Fig.1):

  • CIS-repressing, to be assembled upstream of the target coding sequence.

  • TRANS-repressor, complementary to the CIS-repressing and placed under the control of a different promoter.

CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.




Project3b.png

Fig.1

Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate. </font> How can we test the device?


In order to test and characterize our T-REX device, we developed the following genetic circuit:

Circuit2.jpg



The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.


More details about our work in the Project section.


Acknowledgements


  • [http://www.unibo.it/Portale/default.htm University of Bologna]


LogoUnibo.jpg








  • [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]


Ser In Ar.jpg






  • Cultural Association San Sebastiano
SSebastiano.jpg











Locations of visitors to this page


Up