Team:Bologna/Wetlab

From 2009.igem.org

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Latest revision as of 03:04, 22 October 2009

HOME	TEAM	PROJECT	SOFTWARE	MODELING	WET LAB	PARTS	HUMAN PRACTICE	JUDGING CRITERIA

PARTS CHARACTERIZATION

Circuit characterization.

STORY OF A T-REX

The sad story of a little T-REX who couldn't find his home.

NOTEBOOK

Our wetlab notebook.

PROTOCOLS

Here you can find all our experimental protocols.

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+<a href="https://2009.igem.org/Team:Bologna/Characterization">PARTS CHARACTERIZATION <br><br> <img src="https://static.igem.org/mediawiki/2009/3/34/1429i13504psb1a2y100cgn170esp1%2C4_v1.png" width="115" height="75"></a></a></i></font>
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+Circuit characterization.</i></font>
+<font face="Times New Roman" font size="6"><i>
+<br><br><br>
+<a href="https://2009.igem.org/Team:Bologna/T-REx Story">STORY OF A T-REX<br><br> <img src="https://static.igem.org/mediawiki/2009/f/f8/T-Rex.png" width="115" height="75"></a></a></i></font>
+<font face="Times New Roman" font size="5"><i>
+The sad story of a little T-REX who couldn't find his home.</i></font>
+<font face="Times New Roman" font size="6"><i>
+<br><br><br>
+<a href="https://2009.igem.org/Team:Bologna/Lab-Notebook">NOTEBOOK <br><br> <img src="https://static.igem.org/mediawiki/2009/2/26/Diary123.jpg" width="115" height="75"></a></a></i></font>
+<font face="Times New Roman" font size="5"><i> Our wetlab notebook.</i></font>
+<font face="Times New Roman" font size="6"><i>
+<br><br><br>
+<a href="https://2009.igem.org/Team:Bologna/WetlabProtocols">PROTOCOLS <br><br> <img src="https://static.igem.org/mediawiki/2009/b/b3/Biohazard.png" width="105" height="90"></a></i></font>
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+Here you can find all our experimental protocols.</i></font>
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-!align="center"|[[Team:Bologna|<font color="#ffffff">HOME</font>]]
-!align="center"|[[Team:Bologna/Team|<font color="#ffffff">TEAM</font>]]
-!align="center"|[[Team:Bologna/Project|<font color="#ffffff">PROJECT</font>]]
-!align="center"|[[Team:Bologna/Modeling|<font color="#ffffff">MODELING</font>]]
-!align="center"|[[Team:Bologna/Wetlab|<font color="#ffffff">WETLAB</font>]]
-!align="center"|[[Team:Bologna/Lab-Notebook|<font color="#ffffff">LAB-NOTEBOOK</font>]]
-!align="center"|[[Team:Bologna/Software|<font color="#ffffff">DRY LAB</font>]]
-!align="center"|[[Team:Bologna/Parts|<font color="#ffffff">SUBMITTED PARTS</font>]]
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 <br>
-To test the ratio between the production of an high copy number plasmid (PSB1A2) and a low copy number one (PSB3K3), we assembled two circuits. The open loop GFP circuits are realized with a 1429 promotor and the standard biobrick I13504.
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-[[Image:1429GFP_openloop_lc.png|center|700 px]]
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-PSB1A2 with high copy number plasmid and a low copy number were transformed in DH5alfa bacterial cells according to the standard protocol. One colony from each plate was picked up and let grow overnight in LB medium at 37°C. One milliliter for each of the two samples was collected by O/N cultures and spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition. Finally, images were elaborated with the fluorescence visualization software and these are the results:
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