Team:Bologna/Wetlab

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Finally, images were elaborated with the fluorescence visualization software and these are the results:
Finally, images were elaborated with the fluorescence visualization software and these are the results:
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{|align="center"
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|[[Image:1429i13504psb1a2y100cgn170esp1,4_v1.png|center|thumbnail|385 px|High copy number plasmid (PSB1A2)]]
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|[[Image:1429i13504psb3k3y100cgn170esp1,4_v1.png|center|thumbnail|385 px|Low copy number plasmid (PSB3K3)]]
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Revision as of 14:24, 28 September 2009

ProvaBol2.png
HOME TEAM PROJECT MODELING WETLAB LAB-NOTEBOOK DRY LAB SUBMITTED PARTS HUMAN PRACTICE


To test the ratio between the production of an high copy number plasmid (PSB1A2) and a low copy number one (PSB3K3), we assembled two circuits. The open loop GFP circuits are realized with a 1429 promotor and the standard biobrick I13504.

1429GFP openloop hc.png
1429GFP openloop lc.png


PSB1A2 with high copy number plasmid and a low copy number were transformed in DH5alfa bacterial cells according to the standard protocol.
One colony from each plate was picked up and let grow overnight in LB medium at 37°C. One milliliter for each of the two samples was collected by O/N cultures and spinned at 6000-8000 rpm for three minutes. The supernatant was harvested and the pellet resuspended. Slides were prepared for the fluorescence bacteria image acquisition.
Finally, images were elaborated with the fluorescence visualization software and these are the results:

High copy number plasmid (PSB1A2)
Low copy number plasmid (PSB3K3)