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Team:British Columbia
From 2009.igem.org
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| + | <img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="trafficlight"> | ||
| + | <map name="trafficlight"> | ||
| + | <area shape="circle" coords="774,121,54" alt="Sensors: This links to the pBAD promoters that sense the arabinose." href="green" /> | ||
| + | <area shape="circle" coords="775,236,55" alt="Lock and Key: this controls when the colors are produced" href="yellow" /> | ||
| + | <area shape="circle" coords="774,350,54" alt="The Jammer: this mRNA sequence blocks the key from opening the lock." href="red" /> | ||
| + | </map> | ||
| + | </html> | ||
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| + | [[Image:E_coli_Traffic_Light_General.png|thumb|center|950px|Schematic black-box representation of the E. coli Biosensor that detects various concentration inputs and color outputs. The idea is discrete analog outputs based on a user-specified threshold for each range of concentration.]] | ||
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The Traffic Light is composed of three distinct subparts: | The Traffic Light is composed of three distinct subparts: | ||
Revision as of 05:28, 21 October 2009
The Bacterial Traffic Light
The goal of our project is to construct an Eschericia coli bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.
The Traffic Light is composed of three distinct subparts:
- The pBAD promoter family
- The lock and key riboregulation system
- The Jammer.
