"
Team:British Columbia
From 2009.igem.org
(Difference between revisions)
(→The Bacterial Traffic Light) |
(→The Bacterial Traffic Light) |
||
| Line 7: | Line 7: | ||
<!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] --> | <!-- [[Image:BritishColumbia-Trafficlight.png|center|400px]] --> | ||
<html> | <html> | ||
| - | <img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="trafficlight"> | + | |
| + | <img src="/wiki/images/thumb/f/fc/E_coli_Traffic_Light_General.png/950px-E_coli_Traffic_Light_General.png" width=950 usemap="#trafficlight"> | ||
| + | |||
<map name="trafficlight"> | <map name="trafficlight"> | ||
| - | <area shape="circle" coords="774,121,54" alt="Sensors: This links to the pBAD promoters that sense the arabinose." href="/Team:British_Columbia/pBAD" /> | + | <area shape="circle" |
| - | <area shape="circle" coords="775,236,55" alt="Lock and Key: this controls when the colors are produced" href="/Team:British_Columbia/LockandKey" /> | + | coords="774,121,54" |
| - | <area shape="circle" coords="774,350,54" alt="The Jammer: this mRNA sequence blocks the key from opening the lock." href="/Team:British_Columbia/Jammer" /> | + | alt="Sensors: This links to the pBAD promoters that sense the arabinose." |
| + | title="Sensors: This links to the pBAD promoters that sense the arabinose." | ||
| + | href="/Team:British_Columbia/pBAD" | ||
| + | /> | ||
| + | |||
| + | <area shape="circle" | ||
| + | coords="775,236,55" | ||
| + | alt="Lock and Key: this controls when the colors are produced" | ||
| + | title="Lock and Key: this controls when the colors are produced" | ||
| + | href="/Team:British_Columbia/LockandKey" | ||
| + | /> | ||
| + | |||
| + | <area shape="circle" | ||
| + | coords="774,350,54" | ||
| + | alt="The Jammer: this mRNA sequence blocks the key from opening the lock." | ||
| + | title="The Jammer: this mRNA sequence blocks the key from opening the lock." | ||
| + | href="/Team:British_Columbia/Jammer" | ||
| + | /> | ||
</map> | </map> | ||
</html> | </html> | ||
Revision as of 05:36, 21 October 2009
The Bacterial Traffic Light
The goal of our project is to construct an Eschericia coli bacterial "traffic light", that responds to increasing concentration levels of a given input (in our case, arabinose) with green, yellow, and red fluorescent outputs. Our project is unique because it is an analog biosensor, capable of responding to a continuous variable signal, rather than a binary biosensor that only signals on/off.
The Traffic Light is composed of three distinct subparts:
- The pBAD promoter family
- The lock and key riboregulation system
- The Jammer.
