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Contents 1 Overall project 2 Biosensor 2.1 The Experiments 2.2 Results 3 Logic Gates 3.1 Miscellaneous Data 3.2 Links

Overall project

1. Sensitivity of a biosensor

2. Logic gates

Biosensor

Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.

The Experiments

1. Random mutagenesis of pBAD promoter sequence.
2. Quantification of mutant promoter-driven GFP fluorescence.
3. Sequencing of select mutants.
4. BioBrick design and submission.

Results

Logic Gates

Miscellaneous Data

Biobricks.zip - Fasta file containing every biobrick from Here

Links

http://rna.tbi.univie.ac.at/ - a package of prediction tools for RNA structures; we used RNAfold to annotate the key and lock structures

http://mobyle.pasteur.fr/cgi-bin/portal.py - a set of web-accessible bioinformatics tools including Mfold, which determines 2D RNA structure and draws it

http://frodo.wi.mit.edu/ - Primer3, a primer design program

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