"
Team:British Columbia/Project
From 2009.igem.org
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Sponsor Us! | Biosensor Sensitivity Notebook | Biosensor Logic Gate Notebook | Bibliography |
|---|
Contents |
Overall project
1. Sensitivity of a biosensor
2. Logic gates
Biosensor
Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.
The Experiments
- Random mutagenesis of pBAD promoter sequence.
- Quantification of mutant promoter-driven GFP fluorescence.
- Sequencing of select mutants.
- BioBrick design and submission.
Results
Logic Gates
Miscellaneous Data
Biobricks.zip - Fasta file containing every biobrick from Here [1] - Biobrick digestion engine - enter the name of a plasmid and an insert, and this will show you the product of an EcoRI and PstI digestion/ligation as a FASTA file (suitable for viewing in your favorite program).
Links
http://rna.tbi.univie.ac.at/ - a package of prediction tools for RNA structures; we used RNAfold to annotate the key and lock structures
http://mobyle.pasteur.fr/cgi-bin/portal.py - a set of web-accessible bioinformatics tools including Mfold, which determines 2D RNA structure and draws it
http://frodo.wi.mit.edu/ - Primer3, a primer design program
