Team:Cambridge/Notebook/Week10

From 2009.igem.org

(Difference between revisions)
(Dry Work)
(Threshold Devices)
Line 14: Line 14:
Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.
Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.
 +
 +
Transformation of TOP10 with activator constructs 50, 51, 52 and the part B0015 in preparation for ligation to pigment parts.
{{Template:CambridgeNewPage}}
{{Template:CambridgeNewPage}}

Revision as of 14:09, 22 September 2009


Week 10

Monday

Wet Work

Threshold Devices

Confirmed successful transfer of 40 (pBad followed by GFP) into the low copy plasmid pSB3K3. Transformed 40 in pSB3K3 into arabinose strain.

Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.

Transformation of TOP10 with activator constructs 50, 51, 52 and the part B0015 in preparation for ligation to pigment parts.

Tuesday

Dry Work

Wet Work

Transformants from yesterday put into overnight culture.

Wednesday

Thursday

Friday

Cambridge Sponsor Logo1.pngCambridge Sponsor Logo2.pngCambridge Sponsor Logo3.pngCambridge Sponsor Logo4.pngCambridge Sponsor Logo5.pngCambridge Sponsor Logo8.pngCambridge Sponsor Logo6.pngCambridge Sponsor Logo7.pngCambridge Sponsor Logo9.pngCambridge Sponsor Logo10.pngCambridge Sponsor Logo11.pngCambridge Sponsor Logo12.pngCambridge Sponsor Logo14.pngCambridge Sponsor Logo13.pngCambridge Sponsor Logo15.pngCambridge Sponsor Logo16.pngCambridge Sponsor Logo17.pngCambridge Sponsor Logo18.pngCambridge Sponsor Logo19.pngBmglab.jpg