http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&feed=atom&action=historyTeam:Cambridge/Notebook/Week10 - Revision history2024-03-28T12:56:10ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=76529&oldid=prevMjs88: /* Wet Work */2009-09-25T15:11:42Z<p><span class="autocomment">Wet Work</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The control transformations from yesterday worked, in that no bacteria could be seen on the plates (undiluted and x10 dilution). The transformations with the ligated constructs did not work, however, indicating that the ligation procedure did not work. Today we aim to use an improved protocol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The control transformations from yesterday worked, in that no bacteria could be seen on the plates (undiluted and x10 dilution). The transformations with the ligated constructs did not work, however, indicating that the ligation procedure did not work. Today we aim to use an improved protocol.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">A ligation mix containing just B0015 was also made, marked as NI (no insert) in the freezer. Transformations with the 50, 51, 52 and B0015 ligation mixes were carried out. Because yields of DNA from the gel extraction were so low, 1.5ul of DNA were used in the transformations. </ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=76168&oldid=prevMjs88: /* Friday */2009-09-25T08:56:22Z<p><span class="autocomment">Friday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Friday ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Friday ==</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Wet Work===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The control transformations from yesterday worked, in that no bacteria could be seen on the plates (undiluted and x10 dilution). The transformations with the ligated constructs did not work, however, indicating that the ligation procedure did not work. Today we aim to use an improved protocol.</ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=75695&oldid=prevMjs88: /* Wet Work */2009-09-24T14:39:50Z<p><span class="autocomment">Wet Work</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following the ligation, the constructs 50, 51, 52 ligated to B0015 were transformed into the arabinose strain. As a control, the products of the digest and gel extraction including all of the parts pre-ligation were also transformed into the same strain in order to check whether the ligation had worked.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Following the ligation, the constructs 50, 51, 52 ligated to B0015 were transformed into the arabinose strain. As a control, the products of the digest and gel extraction including all of the parts pre-ligation were also transformed into the same strain in order to check whether the ligation had worked.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The products of the digest and extraction are to be found in Box 1, labelled 50d, 51d, 52d, B0015d 1.5 and B0015d 2.0 (as there were two bands for this part following the digest, both were extracted).</ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=75618&oldid=prevMjs88: /* Thursday */2009-09-24T14:01:07Z<p><span class="autocomment">Thursday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Thursday ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Thursday ==</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Wet Work===</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Following the ligation, the constructs 50, 51, 52 ligated to B0015 were transformed into the arabinose strain. As a control, the products of the digest and gel extraction including all of the parts pre-ligation were also transformed into the same strain in order to check whether the ligation had worked.</ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=75388&oldid=prevMjs88: /* Thursday */2009-09-24T09:51:19Z<p><span class="autocomment">Thursday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Thursday ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Thursday ==</div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=75387&oldid=prevMjs88: /* Wednesday */2009-09-24T09:50:53Z<p><span class="autocomment">Wednesday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out. </div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=75386&oldid=prevMjs88: /* Wednesday */2009-09-24T09:50:19Z<p><span class="autocomment">Wednesday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Wednesday ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Wednesday ==</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Wet Work===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Activator constructs 50, 51, 52 and the part B0015 were mini-prepped from the overnight culture and run on a gel for extraction and purification. Ligations between each of the activators and B0015 were then carried out. </ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=73754&oldid=prevMjs88: /* Threshold Devices */2009-09-22T14:09:56Z<p><span class="autocomment">Threshold Devices</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Confirmed successful construction of pBad + activator (50, 51, and 52) + B0015.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Transformation of TOP10 with activator constructs 50, 51, 52 and the part B0015 in preparation for ligation to pigment parts. </ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=73747&oldid=prevMjs88: /* Dry Work */2009-09-22T14:08:48Z<p><span class="autocomment">Dry Work</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Tuesday ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Tuesday ==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Modelling team gets hands on Matlab. Things may happen!</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===Wet Work===</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Transformants from yesterday put into overnight culture.</ins></div></td></tr>
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</table>Mjs88http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Notebook/Week10&diff=73644&oldid=prevMjs88: /* Tuesday */2009-09-22T09:35:30Z<p><span class="autocomment">Tuesday</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Tuesday ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Tuesday ==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Dry Work===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Modelling team gets hands on Matlab. Things may happen!</ins></div></td></tr>
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