Team:Cambridge/Notebook/Week7

From 2009.igem.org

(Difference between revisions)
(Wet Work)
(Dry Work)
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===Dry Work===
===Dry Work===
 +
We found the phzM and phzS pigments in the registry!
 +
:*BBa_I723024 (phzM)
 +
:*BBa_I723025 (phzS)
 +
 +
This means we can now start working on these pigments, and connecting them too processing systems.
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Revision as of 14:57, 24 August 2009


Week 7

Monday

Wet Work

Amplification

Colony PCR of 8 colonies picked from 391 in pSB3K3 transformants and 8 colonies picked from 374 in pSB3K3 transformants, overnight cultures of each of those colonies as well.

MelA BioBrick

PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted.

We also tested the Primer C again, as it didn't work last time. Three PCR's were done using the same Primer C, a different aliquot of Primer C and re-vortexed Primer C from the origonal vial. None of these worked, so the primer C i have is not working.

Dry Work

We found the phzM and phzS pigments in the registry!

  • BBa_I723024 (phzM)
  • BBa_I723025 (phzS)

This means we can now start working on these pigments, and connecting them too processing systems.

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