Team:Cambridge/Notebook/Week9

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Week 9

Monday

Tuesday

Wednesday

Wet Work

Threshold Devices

Confirmed successful ligation of pBad and I746350, I746351, I746352.

Thursday

Wet Work

Threshold Devices

Attempted the following standard assemblies:

pBad + I746350 to B0015
pBad + I746351 to B0015
pBad + I746352 to B0015
I746351 to B0015
I746352 to B0015
I746352 to B0015

The first three will be used to construct a complete device, with pBad as the sensor promoter and a pigment operon as the pigment-generating device. The pigment we chose for our proof of concept will be placed downstream of each of the 5 phage promoters, and then combined to give 15 combinations of activators and promoters, giving the construction below. We still need to decide which pigment to use for our proof of concept. Completedevice.jpg


The second three will be used to construct a library of threshold devices which can be abstracted to black boxes with PoPS in and PoPS out:

Thresholddevice.jpg

Friday

Cambridge Sponsor Logo1.pngCambridge Sponsor Logo2.pngCambridge Sponsor Logo3.pngCambridge Sponsor Logo4.pngCambridge Sponsor Logo5.pngCambridge Sponsor Logo8.pngCambridge Sponsor Logo6.pngCambridge Sponsor Logo7.pngCambridge Sponsor Logo9.pngCambridge Sponsor Logo10.pngCambridge Sponsor Logo11.pngCambridge Sponsor Logo12.pngCambridge Sponsor Logo14.pngCambridge Sponsor Logo13.pngCambridge Sponsor Logo15.pngCambridge Sponsor Logo16.pngCambridge Sponsor Logo17.pngCambridge Sponsor Logo18.pngCambridge Sponsor Logo19.pngBmglab.jpg