http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&feed=atom&action=historyTeam:Cambridge/Project/Pigments - Revision history2024-03-29T15:12:47ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159574&oldid=prevVmullin: /* Choosing Pigments */2009-10-21T23:26:49Z<p><span class="autocomment">Choosing Pigments</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png|700px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png|700px]]</div></td></tr>
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</table>Vmullinhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159560&oldid=prevVmullin: /* Choosing Pigments */2009-10-21T23:26:25Z<p><span class="autocomment">Choosing Pigments</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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</table>Vmullinhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159502&oldid=prevVmullin: /* Colour Generators */2009-10-21T23:24:47Z<p><span class="autocomment">Colour Generators</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:SDC105451.JPG|<del class="diffchange diffchange-inline">600px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:SDC105451.JPG|<ins class="diffchange diffchange-inline">400px</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td></tr>
</table>Vmullinhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159457&oldid=prevVmullin: /* The Kit of Parts */2009-10-21T23:23:35Z<p><span class="autocomment">The Kit of Parts</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:SDC105451.JPG|600px]]</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Cam09_vio.jpg|300px]][[Image:Cam09_gre.jpg|300px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Cam09_vio.jpg|300px]][[Image:Cam09_gre.jpg|300px]]</div></td></tr>
</table>Vmullinhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159132&oldid=prevMsmsimon: /* The Kit of Parts */2009-10-21T23:13:50Z<p><span class="autocomment">The Kit of Parts</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Cam09_vio.jpg|300px]][[Image:Cam09_gre.jpg|300px]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As as a result of our efforts, this summer, we have submitted the following biobricks to the Registry. We hope that in the future this this kit of parts will be expanded in the future.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As as a result of our efforts, this summer, we have submitted the following biobricks to the Registry. We hope that in the future this this kit of parts will be expanded in the future.</div></td></tr>
</table>Msmsimonhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159118&oldid=prevMsmsimon: /* The Kit of Parts */2009-10-21T23:13:21Z<p><span class="autocomment">The Kit of Parts</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Cambridge 2009 iGEM team searched far and wide to find suitable pigments that could be used as outputs. Though ''E. coli'' does not naturally produce any pigment, several other bacterial species secrete pigmented antibiotics. We mined bacterial genomes for pigment-producing operons, and transformed the most prominent candidates into ''E. coli''. In particular, we have devoted our summer to 3 different pigment systems:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Cambridge 2009 iGEM team searched far and wide to find suitable pigments that could be used as outputs. Though ''E. coli'' does not naturally produce any pigment, several other bacterial species secrete pigmented antibiotics. We mined bacterial genomes for pigment-producing operons, and transformed the most prominent candidates into ''E. coli''. In particular, we have devoted our summer to 3 different pigment systems:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/CA01 |Carotenoids]]''': The enzymes required for carotenoid production originally come from ''Pantoea ananatis,'' and were available in the registry. We used them to produce orange and red.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/CA01 |Carotenoids]]''': The enzymes required for carotenoid production originally come from ''Pantoea ananatis,'' and were available in the registry. We used them to produce orange and red.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Cam09_ca1.jpg|300px]][[Image:Cam09_ca2.jpg|300px]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td></tr>
</table>Msmsimonhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=159061&oldid=prevVmullin: /* Choosing Pigments */2009-10-21T23:10:43Z<p><span class="autocomment">Choosing Pigments</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png|700px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png|700px]]</div></td></tr>
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</table>Vmullinhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=158569&oldid=prevMsmsimon: /* Choosing Pigments */2009-10-21T22:56:56Z<p><span class="autocomment">Choosing Pigments</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We chose to pursue these 3 pigment systems for two main reasons.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png|<ins class="diffchange diffchange-inline">700px</ins>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png|<del class="diffchange diffchange-inline">500px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Design''': We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Design''': We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.</div></td></tr>
</table>Msmsimonhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=158554&oldid=prevMsmsimon: /* Choosing Pigments */2009-10-21T22:56:29Z<p><span class="autocomment">Choosing Pigments</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:RAINBOW.png<ins class="diffchange diffchange-inline">|500px</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Design''': We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Design''': We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.</div></td></tr>
</table>Msmsimonhttp://2009.igem.org/wiki/index.php?title=Team:Cambridge/Project/Pigments&diff=158535&oldid=prevMsmsimon at 22:56, 21 October 20092009-10-21T22:56:02Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 22:56, 21 October 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/ME01 |Melanin]]''': The tyrosinase required for melanin production originally comes from ''Rhizobium etli'' and produces brown.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*'''[[Team:Cambridge/Project/VI01 |Violacein]]''': The enzymes required for voilacein production originally come from ''Chromobacterium violacein.'' The operon can be manipulated to produce voilet, green, and blue.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:RAINBOW.png]]</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As as a result of our efforts, this summer, we have submitted the following biobricks to the Registry. We hope that in the future this this kit of parts will be expanded in the future.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As as a result of our efforts, this summer, we have submitted the following biobricks to the Registry. We hope that in the future this this kit of parts will be expanded in the future.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Visual Diversity''': Not only do these devices come from different bacterial species, but between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">A COOL PICTURE HERE</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:RAINBOW.png]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Design''': We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Design''': We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.</div></td></tr>
</table>Msmsimon