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Team:Cambridge/Protocols - Revision history
2024-03-28T13:34:19Z
Revision history for this page on the wiki
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http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=70257&oldid=prev
Awalbridge: /* Protocols */
2009-09-17T15:29:33Z
<p><span class="autocomment">Protocols</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:29, 17 September 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Carotene extraction with acetone | Carotene Extraction ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Carotene extraction with acetone | Carotene Extraction ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | Finnzymes <del class="diffchange diffchange-inline">Phusion </del>PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | Finnzymes PCR]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td></tr>
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Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=70254&oldid=prev
Awalbridge: /* Protocols */
2009-09-17T15:29:06Z
<p><span class="autocomment">Protocols</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:29, 17 September 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Carotene extraction with acetone | Carotene Extraction ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Carotene extraction with acetone | Carotene Extraction ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | Finnzymes Phusion <del class="diffchange diffchange-inline">(TM) </del>PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | Finnzymes Phusion PCR]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td></tr>
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Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=70253&oldid=prev
Awalbridge: /* Protocols */
2009-09-17T15:28:45Z
<p><span class="autocomment">Protocols</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:28, 17 September 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#PCR procedures | PCR Procedures]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#PCR procedures | PCR Procedures]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Glycerol Stocks | Glycerol Stocks]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Glycerol Stocks | Glycerol Stocks]]</div></td></tr>
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Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=70252&oldid=prev
Awalbridge: /* Protocols */
2009-09-17T15:27:34Z
<p><span class="autocomment">Protocols</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Carotene extraction with acetone | Carotene Extraction ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Carotene extraction with acetone | Carotene Extraction ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | <ins class="diffchange diffchange-inline">Finnzymes Phusion (TM) </ins>PCR]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatetop}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatetop}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#PCR procedures | PCR Procedures]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#PCR procedures | PCR Procedures]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[#Glycerol <del class="diffchange diffchange-inline">Stock </del>| Glycerol</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[#Glycerol <ins class="diffchange diffchange-inline">Stocks </ins>| Glycerol <ins class="diffchange diffchange-inline">Stocks]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td></tr>
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</table>
Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=70249&oldid=prev
Awalbridge: /* Protocols */
2009-09-17T15:26:41Z
<p><span class="autocomment">Protocols</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:26, 17 September 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Agarose gel electrophoresis | Electrophoresis ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Finnzymes Phusion (TM) PCR | PCR]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Template:Cambridgetemplatebottom}}</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#PCR procedures | PCR Procedures]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#PCR procedures | PCR Procedures]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Glycerol Stock | Glycerol</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#Glycerol Stock | Glycerol</div></td></tr>
</table>
Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=68139&oldid=prev
Awalbridge at 09:42, 15 September 2009
2009-09-15T09:42:17Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:42, 15 September 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridge2}}<!--Do not remove the first and last lines in this page!--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridge2}}<!--Do not remove the first and last lines in this page!--></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">= Protocols =</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- This is for the top grey / blue links bar !--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- This is for the top grey / blue links bar !--></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:Cambridgetemplatebottom}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">= Protocols =</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Producing competent cells ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Producing competent cells ==</div></td></tr>
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Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=68138&oldid=prev
Awalbridge at 09:41, 15 September 2009
2009-09-15T09:41:43Z
<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#Producing competent cells | Competent Cells]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#Competent cells Transformation | Transformation ]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#Carotene extraction with acetone | Carotene Extraction ]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#Agarose gel electrophoresis | Electrophoresis ]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#Finnzymes Phusion (TM) PCR | PCR]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#PCR procedures | PCR Procedures]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[#Glycerol Stock | Glycerol</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Template:Cambridgetemplatebottom}}</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Protocols =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Protocols =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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Awalbridge
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=50616&oldid=prev
Vmullin: /* PCR procedures */
2009-08-20T10:16:51Z
<p><span class="autocomment">PCR procedures</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 10:16, 20 August 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*Eco-Taq: 0.2 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*Eco-Taq: 0.2 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*10X buffer: 5 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*10X buffer: 5 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:*dNTPs: 0.4 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*dNTPs: 0.4 uL <ins class="diffchange diffchange-inline">(stock is 10 mM)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*make up to 20 uL volume with H20</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*make up to 20 uL volume with H20</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Reaction procedure'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Reaction procedure'''</div></td></tr>
</table>
Vmullin
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=50609&oldid=prev
Vmullin: /* PCR procedures */
2009-08-20T09:54:11Z
<p><span class="autocomment">PCR procedures</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 09:54, 20 August 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 151:</td>
<td colspan="2" class="diff-lineno">Line 151:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*95 degrees C for 2 minutes</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*95 degrees C for 2 minutes</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*33 cycles of:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*33 cycles of:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*95 degrees C for 30 seconds (denaturation)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:<ins class="diffchange diffchange-inline">:</ins>*95 degrees C for 30 seconds (denaturation)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*65 degrees C for 30 seconds (annealing)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:<ins class="diffchange diffchange-inline">:</ins>*65 degrees C for 30 seconds (annealing)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*72 degrees C, 1000bp/min (elongation)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:<ins class="diffchange diffchange-inline">:</ins>*72 degrees C, 1000bp/min (elongation)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*72 degrees C for 5 minutes</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*72 degrees C for 5 minutes</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*hold at 4 degrees C</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*hold at 4 degrees C</div></td></tr>
</table>
Vmullin
http://2009.igem.org/wiki/index.php?title=Team:Cambridge/Protocols&diff=50608&oldid=prev
Vmullin: /* PCR procedures */
2009-08-20T09:53:52Z
<p><span class="autocomment">PCR procedures</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 09:53, 20 August 2009</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 139:</td>
<td colspan="2" class="diff-lineno">Line 139:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*20ul solution</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*20ul solution</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Run with standard procedure as follows (from James) for Colony PCR</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Run with standard procedure as follows (from James) for Colony PCR</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">:</del>*Reaction Mixture</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*Template: 1 uL from O/N culture (1-2 uL if colony picked straight from plate into water)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">'''</ins>Reaction Mixture<ins class="diffchange diffchange-inline">'''</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*VF2: 1uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*Template: 1 uL from O/N culture (1-2 uL if colony picked straight from plate into water)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*VR: 1uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*VF2: 1uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*Eco-Taq: 0.2 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*VR: 1uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*10X buffer: 5 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*Eco-Taq: 0.2 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*dNTPs: 0.4 uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*10X buffer: 5 uL</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*dNTPs: 0.4 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*make up to 20 uL volume with H20</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*make up to 20 uL volume with H20</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">:</del>*Reaction procedure</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">'''</ins>Reaction procedure<ins class="diffchange diffchange-inline">'''</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*95 degrees C for 2 minutes</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*95 degrees C for 2 minutes</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*33 cycles of:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*33 cycles of:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>**95 degrees C for 30 seconds (denaturation)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:**95 degrees C for 30 seconds (denaturation)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>**65 degrees C for 30 seconds (annealing)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:**65 degrees C for 30 seconds (annealing)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>**72 degrees C, 1000bp/min (elongation)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:**72 degrees C, 1000bp/min (elongation)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*72 degrees C for 5 minutes</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*72 degrees C for 5 minutes</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:<del class="diffchange diffchange-inline">*</del>*hold at 4 degrees C</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:*hold at 4 degrees C</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Gels'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Gels'''</div></td></tr>
</table>
Vmullin