Team:Chiba/Project
From 2009.igem.org
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+ | '''Constructing A Delay Switch''' | ||
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+ | 去年の紹介 | ||
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+ | [[Team:Chiba/Project/LuxR Mutants|LuxR Mutants]] | ||
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Revision as of 08:33, 2 September 2009
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IntroductionProject DesignSignaling SystemIn this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. (more about quorum sensing)
Constructing A Delay Switch, Multiple Ways2008のことを述べ、Mutantの実験をやってない(今年はやる)とせつめい。 In principle, there are three ways to delay the activation of chemical communications;
時限装置の集合体で時間の流れを表す1, Parallel Communication
2, Series communicationConclusions |