Team:Chiba/Project/Las Communication Check

Existence of LVA might inhibit the activity of the protein by physically blocking its forming　functional　architecture or by excessively　shorten the half-life of the protein, the function of this tag, and let us be unable to find.

@@ Line 7: / Line 7: @@
 <table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
 <tr><td>
-[[Team:Chiba|Home]]
+'''[[Team:Chiba|Home]]'''
 </td></tr><tr><td>
 '''[[Team:Chiba/Project|The Project]]'''
 </td></tr><tr><td>
-Introduction
+*[[Team:Chiba/Project#Introduction|Introduction]]
 </td></tr><tr><td>
-Project Design
+*[[Team:Chiba/Project#Project_Design|Project Design]]
 </td></tr><tr><td>
-Conclusions
+*[[Team:Chiba/Project#Conclusions|Conclusions]]
 </td></tr></table>
@@ Line 21: / Line 21: @@
 <table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
 <tr><td>
-'''Constructing A Delay Switch'''
+'''[[Team:Chiba/Project/Delay_Switch|Delay Switch]]'''
 </td></tr><tr><td>
-*去年の紹介
+*[[Team:Chiba/Project/Delay_Switch#Experiments|Experiments]]
 </td></tr><tr><td>
-*[[Team:Chiba/Project/LuxR Mutants|LuxR Mutants]]
+*[[Team:Chiba/Project/Delay_Switch#Results|Results]]
 </td></tr></table>
@@ Line 31: / Line 31: @@
 <table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
 <tr><td>
-'''Parallel communication'''
+'''[[Team:Chiba/Project/Parallel|Parallel Activation]]'''
-</td></tr><tr><td>
-*[[Team:Chiba/Project/Parallel|Overall]]
-</td></tr><tr><td>
-*[[Team:Chiba/Project/LuxR Mutants|LuxR Mutants]]
 </td></tr><tr><td>
 *[[Team:Chiba/Modeling|Modeling]]
 </td></tr><tr><td>
-*Testing
+*[[Team:Chiba/Project/Parallel#Experiments|Experiments]]
 </td></tr><tr><td>
-*Results
+*[[Team:Chiba/Project/Parallel#Results|Results]]
 </td></tr></table>
@@ Line 47: / Line 43: @@
 <table width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
 <tr><td>
-'''Series communication'''
+'''[[Team:Chiba/Project/Series|Series Activation]]'''
-</td></tr><tr><td>
-*[[Team:Chiba/Project/Series|Overall]]
 </td></tr><tr><td>
 *[[Team:Chiba/Project/Las Communication Check|Las Check]]
@@ Line 55: / Line 49: @@
 *[[Team:Chiba/Modeling|Modeling]]
 </td></tr><tr><td>
-*Testing
+*[[Team:Chiba/Project/Series#Experiments|Experiments]]
 </td></tr><tr><td>
-*Results
+*[[Team:Chiba/Project/Series#Results|Results]]
 </td></tr></table>
 |style="width:85%;" bgcolor="#FFFFFF"|
@@ Line 71: / Line 66: @@
 !align="center"|[[Team:Chiba/Contact|Contact]]
 |}
+__NOTOC__
+=== Las Communication Check ===
+昨年のプロジェクトでは、LasRの稼動が確認できなかった。
-===Las Check===
+Last year, our team tried to use some circuit containing LasR with LVA tag(BBa_C0079). However we could not find out the activation of the parts.
-昨年のプロジェクトでは、LasRの稼動が確認できなかった。
 iGEMのページを見ても、使ったパーツが動いたとの記述が見当たらない。
+In addition, we also have not found the report that the parts(BBa_C0079) had surely operated.
 本当に動くのかを確認する。
+So we will check the activity of LasR this year.
 ・LVAタグの存在
+・Existence of LVA tag
 タグが付いていることによって酵素活性が阻害されている可能性がある。
 タンパク質の高次構造の形成を物理的に阻害しているかもしれない。
-機能を弱める（抑制？分解？）機能を持ったタグなので、そのせいで活性が下がっていて稼動していないように見えるのかもしれない。
+機能を弱める（抑制？分解？）働きを持ったタグなので、そのせいで活性が下がっていて稼動していないように見えるのかもしれない。
+Existence of LVA might inhibit the activity of the protein by physically blocking its forming　functional　architecture or by excessively　shorten the half-life of the protein, the function of this tag, and let us be unable to find.