Team:Cornell/Parts

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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[[Image:CUGEM_Banner.png|center|800px]]
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Example_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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{| style="border:1px solid #cef2e0; background:#f5fffa; color:#008811; vertical-align:top;" cellpadding="3" cellspacing="1" width="900px" align="center"
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|[[Image:Team.png|right|frame|Your team picture]]
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!align="center"|[[Team:Cornell|The Project]]
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!align="center"|[[Team:Cornell/Project/Background|Background]]
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!align="center"|[[Team:Cornell/Project/Chassis|Chassis]]
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|align="center"|[[Team:Cornell | Team Example]]
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!align="center"|[[Team:Cornell/Project/Design|Design]]
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!align="center"|[[Team:Cornell/Project/Standardization|Standardization]]
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!align="center"|[[Team:Cornell/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Cornell/Notebook|Notebook]]
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!align="center"|[[Team:Cornell/Team|The Team]]
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=Parts=
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===Device===
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''Cadmium Sensor (BBa_K285104)''
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expresses GFP as a function of Cadmium Ion concentration. The first part of the GFP Cadmium Sensor is the CadA regulatory element which increases expression in response increasing Cadmium concentration. This is followed by spoVG a ribosome binding site for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis.
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[[Image:Module 1 Active.GIF|600px|center]]
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<!--- The Mission, Experiments --->
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=== Individual Parts===
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''CadA Regulatory Region (BBa_K285101,[http://partsregistry.org/Part:BBa_K285101:Design])'' is the regulatory sequence upstream of the cadA gene (previously known as yvgW). This sequence is part of the czrA regulon and is regulated by the CzrA repressor which is derepressed in the presence of Cd2+.[1][2]
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''copZA Regulatory Region (BBa_K285102,[http://partsregistry.org/Part:BBa_K285102:Design])'' is the regulatory sequence upstream of the copZ and copA genes. Activation of the copZ gene proceeds by copper mediated induction by the CueR protein which binds to this region.[2]
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!align="center"|[[Team:Cornell|Home]]
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!align="center"|[[Team:Cornell/Team|The Team]]
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''mntH Regulatory Region (BBa_K285103,[http://partsregistry.org/Part:BBa_K285103:Design])''is upstream of the mntH gene is repressed by the MntR protein which is derepressed upon induction by Cd2+.[1][3]
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!align="center"|[[Team:Cornell/Project|The Project]]
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!align="center"|[[Team:Cornell/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Cornell/Modeling|Modeling]]
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!align="center"|[[Team:Cornell/Notebook|Notebook]]
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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=== References===
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[1]Moore CM, Gaballa A, Hui M, Ye RW, Helmann JD. Genetic and physiological responses of Bacillus subtilis to metal ion stress. Mol Microbiol. 2005 Jul;57(1):27-40.
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===Note===
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[2]Ahmed Gaballa & John D. Helmann Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems. Biometals. 2003 Dec;16(4):497-505.
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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[3]Que Q, Helmann JD.Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins.Mol Microbiol. 2000 Mar;35(6):1454-68.

Latest revision as of 03:58, 22 October 2009

CUGEM Banner.png
The Project Background Chassis Design Standardization Parts Submitted to the Registry Notebook The Team

Contents

Parts

Device

Cadmium Sensor (BBa_K285104) expresses GFP as a function of Cadmium Ion concentration. The first part of the GFP Cadmium Sensor is the CadA regulatory element which increases expression in response increasing Cadmium concentration. This is followed by spoVG a ribosome binding site for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis.

Module 1 Active.GIF

Individual Parts

CadA Regulatory Region (BBa_K285101,[1]) is the regulatory sequence upstream of the cadA gene (previously known as yvgW). This sequence is part of the czrA regulon and is regulated by the CzrA repressor which is derepressed in the presence of Cd2+.[1][2]

copZA Regulatory Region (BBa_K285102,[2]) is the regulatory sequence upstream of the copZ and copA genes. Activation of the copZ gene proceeds by copper mediated induction by the CueR protein which binds to this region.[2]

mntH Regulatory Region (BBa_K285103,[3])is upstream of the mntH gene is repressed by the MntR protein which is derepressed upon induction by Cd2+.[1][3]

References

[1]Moore CM, Gaballa A, Hui M, Ye RW, Helmann JD. Genetic and physiological responses of Bacillus subtilis to metal ion stress. Mol Microbiol. 2005 Jul;57(1):27-40.

[2]Ahmed Gaballa & John D. Helmann Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems. Biometals. 2003 Dec;16(4):497-505.

[3]Que Q, Helmann JD.Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins.Mol Microbiol. 2000 Mar;35(6):1454-68.

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