Team:Cornell/Parts

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Device

Cadmium Sensor (BBa_K285104) expresses GFP as a function of Cadmium Ion concentration. The first part of the GFP Cadmium Sensor is the CadA regulatory element which increases expression in response increasing Cadmium concentration. This is followed by spoVG a ribosome binding site for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis.

Module 1 Active.GIF


Individual Parts

CadA Regulatory Region (BBa_K285101) is

copZA Regulatory Region (BBa_K285102) is bound by CueR protein in the presence of Copper activating the copZ gene. The copZA regulatory region is previously known as yvgY.

mntH Regulatory Region (BBa_K285103)is upstream of the mntH gene is repressed by the MntR protein which is derepressed upon induction by Cd2+.