Team:EPF-Lausanne

From 2009.igem.org

(Difference between revisions)
(Concept)
Line 7: Line 7:
==Concept==
==Concept==
<div style="text-align:justify;">
<div style="text-align:justify;">
-
[[Image:LovTAP_dimer.png|right|300px|LovTAP dimer bound to DNA]]
+
[[Image:LovTAP_dimer.png|right|300px|thumb|LovTAP dimer bound to DNA]]
Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
Line 16: Line 16:
</div>
</div>
-
 
+
<br><br>
{| class="wikitable" style="text-align:center; width:100%;"
{| class="wikitable" style="text-align:center; width:100%;"
|-
|-

Revision as of 22:43, 21 October 2009

Mainpage.jpg



Concept

LovTAP dimer bound to DNA

Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.

For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.

The results clearly show that this light-induced gene switch tool works in vivo, demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.



Logo MerckSerono.png Logo Novartis.png Logo Syngenta.png Logo UBS.jpg Logo ciba.jpg Logo nikon.jpg Logo tecan.gif

Locations of visitors to this page