Team:EPF-Lausanne/Notebook/Cloning Strategy

From 2009.igem.org

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(New page: ;Cloning strategy: Four forward primers were designed to amplify: <br> 1.Promoter T7, RBS, CBP and LOVTAP: :gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg 2.RBS, CBP and LOVT...)
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;Cloning strategy:
 
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Four forward primers were designed to amplify:
 
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<br> 1.Promoter T7, RBS, CBP and LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg
 
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2.RBS, CBP and LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag
 
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3.CBP and LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag
 
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4.LOVTAP:
 
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:gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac
 
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One reverse primer were designed:
 
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:gtttcttcctgcagcggccgctactagtatcaatcgcttttcagcaacacctcttc
 
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The '''recipient IGEM part''' have been chosen: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_B0010 BBa_B0010], well 13D in the received kit plate 1
 

Latest revision as of 08:05, 28 July 2009

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