Team:EPF-Lausanne/Protocols/Transformation

From 2009.igem.org

Transformation




Material

  • 50 µl competent cells
  • LB-agar plates with corresponding antibiotics
  • Water bath at 42°C


Procedure

1. Thaw cells on ice for 20min
2. Add DNA (ligation product) to the cells, mix.
3. Incubate 20min on ice
4. Heat shock 45s @ 42°C
5. Incubate 20min on ice (optional)
6. Add 500 µl SOC and incubate 1h @ 37°C (shaking)
7. Spread over LB-agar plate
8. Incubate overnight @ 37°C