Team:Freiburg bioware

From 2009.igem.org

(Difference between revisions)
Line 1: Line 1:
-
[[Image:Header3.jpg]]
+
{{:Team:Freiburg_bioware/Template1}}
-
<br>
+
-
<b>Project Abstract</b>
+
-
----
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
-
 
+
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en"
-
 
+
dir="ltr" lang="en-US">
-
After the second place in last year’s iGEM competition we got high expectations and look forward to this year’s Jamboree! We’re working on the development of an universal restriction enzyme to facilitate labwork and enable new techniques.
+
<head>
-
<br>  
+
  <meta http-equiv="Content-Type"
-
<br>
+
content="text/html; charset=UTF-8" />
-
Restriction enzymes are proteins capable of cutting double or single strand nuclein acids. We focus on type II and III restriction enzymes which at first bind the DNA strand, then recognize a certain sequence pattern where they cut the DNA backbone. There are thousands of different restriction enzymes, each with particular recognition and cutting sites. These enzymes are essential tools for gene cloning and protein expression experiments. Every medical and biological laboratory needs to keep dozens of different restriction enzymes in stock for regular use. Acquiring and handling so many enzymes is very expensive and time consuming. We are determined to simplify this procedure totally, by creating one enzyme for every occasion.  
+
  <meta http-equiv="X-UA-Compatible" content="IE=EmulateIE7" />
-
<br>  
+
  <title>FREiGEM 2009</title>
-
<br>
+
  <script type="text/javascript" src="script.js"></script>
-
One of the biggest challenges of today is to cure diseases by means of gene therapy. The aim here is to artifically introduce genetic information in somatic cells to substitute DNA-sequences which may allow the correction of mutated genes. Particularly in monogenetic diseases this would lead to a change of the phenotype. The human genome contains 3×10^9 bp which code for approximately 30.000 different genes. Alone one single mutation can cause changes which may lead to diseases or even death. Because of this it is tried in gene therapy to address exactly these mutations. But this means that it is necessary to cause specifically on that point a change e.g. by cutting. Restriction enzymes could be used as high specific tools for this. But these enzymes would have to recognize a sequence of at least 16 bp in oder to cut only once in the human genome (416 bp = 4.3*10^9 bp). Most of the known 3500 restriction enzymes only recognize sequences with a length of 4-8 bp. One application of our work could be to construct an artificial restriction enzyme with a recognition sequence of at least 16 bp of length and which is programmable for many different target sequences.
+
  <link rel="stylesheet" href="style2.css"
-
 
+
type="text/css" media="screen" />
-
 
+
<!--[if IE 6]><link rel="stylesheet" href="style.ie6.css" type="text/css" media="screen" /><![endif]--><!--[if IE 7]><link rel="stylesheet" href="style.ie7.css" type="text/css" media="screen" /><![endif]-->
-
 
+
</head>
-
 
+
<body>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<div id="art-page-background-simple-gradient"> </div>
-
!align="center"|[[Team:Freiburg_bioware|Home]]
+
<div id="art-main">
-
!align="center"|[[Team:Freiburg_bioware/Team|The Team]]
+
<div class="art-Sheet">
-
!align="center"|[[Team:Freiburg_bioware/Project|The Project]]
+
<div class="art-Sheet-tl"></div>
-
!align="center"|[[Team:Freiburg_bioware/Parts|Parts Submitted to the Registry]]
+
<div class="art-Sheet-tr"></div>
-
!align="center"|[[Team:Freiburg_bioware/Modeling|Modeling]]
+
<div class="art-Sheet-bl"></div>
-
!align="center"|[[Team:Freiburg_bioware/Notebook|Notebook]]
+
<div class="art-Sheet-br"></div>
-
|}
+
<div class="art-Sheet-tc"></div>
-
 
+
<div class="art-Sheet-bc"></div>
-
 
+
<div class="art-Sheet-cl"></div>
-
<html><center>
+
<div class="art-Sheet-cr"></div>
-
<a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Freiburg_bioware" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Freiburg_bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" />
+
<div class="art-Sheet-cc"></div>
-
</a>
+
<div class="art-Sheet-body">
-
</center></html>
+
<div class="art-Header">
 +
<div class="art-Header-png"></div>
 +
<div class="art-Header-jpeg"></div>
 +
</div>
 +
<div class="art-nav">
 +
<div class="l"></div>
 +
<div class="r"></div>
 +
<ul class="art-menu">
 +
  <li><a href="#" class="active"><span
 +
class="l"></span><span class="r"></span><span
 +
class="t">Home</span></a></li>
 +
  <li><a href="#"><span class="l"></span><span
 +
class="r"></span><span class="t">The Team</span></a>
 +
    <ul>
 +
      <li><a href="#">Fotos</a></li>
 +
      <li><a href="#">Fotos</a></li>
 +
    </ul>
 +
  </li>
 +
  <li><a href="#"><span class="l"></span><span
 +
class="r"></span><span class="t">The
 +
Project</span></a></li>
 +
  <li><a href="#"><span class="l"></span><span
 +
class="r"></span><span class="t">Parts</span></a>
 +
    <ul>
 +
      <li><a href="#">Link 1</a>
 +
        <ul>
 +
          <li><a href="#">Link 1a</a> </li>
 +
          <li><a href="#">Link 1b</a> </li>
 +
          <li><a href="#">Link 1c</a> </li>
 +
        </ul>
 +
      </li>
 +
      <li><a href="#">Link 2</a></li>
 +
      <li><a href="#">Link 3</a></li>
 +
    </ul>
 +
  </li>
 +
  <li><a href="#"><span class="l"></span><span
 +
class="r"></span><span class="t">Notebook</span></a></li>
 +
</ul>
 +
</div>
 +
<div class="art-contentLayout">
 +
<div class="art-content">
 +
<div class="art-Post">
 +
<div class="art-Post-tl"></div>
 +
<div class="art-Post-tr"></div>
 +
<div class="art-Post-bl"></div>
 +
<div class="art-Post-br"></div>
 +
<div class="art-Post-tc"></div>
 +
<div class="art-Post-bc"></div>
 +
<div class="art-Post-cl"></div>
 +
<div class="art-Post-cr"></div>
 +
<div class="art-Post-cc"></div>
 +
<div class="art-Post-body">
 +
<div class="art-Post-inner">
 +
<div class="art-PostMetadataHeader">
 +
<h2 class="art-PostHeaderIcon-wrapper"> &nbsp;<span
 +
class="art-PostHeader">Welcome</span> </h2>
 +
</div>
 +
<div class="art-PostContent"> <img class="art-article"
 +
src="https://static.igem.org/mediawiki/2009/6/62/Freiburg09_Ruedigerlogo.png"
 +
alt="" style="width: 205px; height: 205px; float: left;" />
 +
<h1>Team Freiburg Bioware</h1>
 +
The first German team ever to participate in iGEM is back again and
 +
after last year's second place we're highly motivated &nbsp;to make
 +
some good peace of synthetic biology in 2009. In this year we want to
 +
favour you with an universal restriction enzyme to facilitate labwork
 +
and enable new techniques. <br />
 +
<br />
 +
We're looking forward to meet you on this year's jamboree!<br />
 +
<br />
 +
<br />
 +
</div>
 +
<div class="cleared"></div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="art-Post">
 +
<div class="art-Post-tl"></div>
 +
<div class="art-Post-tr"></div>
 +
<div class="art-Post-bl"></div>
 +
<div class="art-Post-br"></div>
 +
<div class="art-Post-tc"></div>
 +
<div class="art-Post-bc"></div>
 +
<div class="art-Post-cl"></div>
 +
<div class="art-Post-cr"></div>
 +
<div class="art-Post-cc"></div>
 +
<div class="art-Post-body">
 +
<div class="art-Post-inner">
 +
<div class="art-PostMetadataHeader">
 +
<h2 class="art-PostHeaderIcon-wrapper"> &nbsp;Project
 +
Summary<span class="art-PostHeader"></span> </h2>
 +
</div>
 +
<div class="art-PostContent">
 +
<p>Restriction enzymes are proteins capable of cutting double or
 +
single
 +
strand nuclein acids. We focus on type II and III restriction enzymes
 +
which at first bind the DNA strand, then recognize a certain sequence
 +
pattern where they cut the DNA backbone. There are thousands of
 +
different restriction enzymes, each with particular recognition and
 +
cutting sites. These enzymes are essential tools for gene cloning and
 +
protein expression experiments. Every medical and biological laboratory
 +
needs to keep dozens of different restriction enzymes in stock for
 +
regular use. Acquiring and handling so many enzymes is very expensive
 +
and time consuming. We are determined to simplify this procedure
 +
totally, by creating one enzyme for every occasion. <br />
 +
<br />
 +
One of the biggest challenges of today is to cure diseases by means of
 +
gene therapy. The aim here is to artifically introduce genetic
 +
information in somatic cells to substitute DNA-sequences which may
 +
allow the correction of mutated genes. Particularly in monogenetic
 +
diseases this would lead to a change of the phenotype. The human genome
 +
contains 3&times;10^9 bp which code for approximately 30.000
 +
different genes.
 +
Alone one single mutation can cause changes which may lead to diseases
 +
or even death. Because of this it is tried in gene therapy to address
 +
exactly these mutations. But this means that it is necessary to cause
 +
specifically on that point a change e.g. by cutting. Restriction
 +
enzymes could be used as high specific tools for this. But these
 +
enzymes would have to recognize a sequence of at least 16 bp in oder to
 +
cut only once in the human genome (416 bp = 4.3*10^9 bp). Most of the
 +
known 3500 restriction enzymes only recognize sequences with a length
 +
of 4-8 bp. One application of our work could be to construct an
 +
artificial restriction enzyme with a recognition sequence of at least
 +
16 bp of length and which is programmable for many different target
 +
sequences.
 +
</p>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="art-sidebar1">
 +
<div class="art-Block">
 +
<div class="art-Block-tl"></div>
 +
<div class="art-Block-tr"></div>
 +
<div class="art-Block-bl"></div>
 +
<div class="art-Block-br"></div>
 +
<div class="art-Block-tc"></div>
 +
<div class="art-Block-bc"></div>
 +
<div class="art-Block-cl"></div>
 +
<div class="art-Block-cr"></div>
 +
<div class="art-Block-cc"></div>
 +
<div class="art-Block-body">
 +
<div class="art-BlockHeader">
 +
<div class="l"></div>
 +
<div class="r"></div>
 +
<div class="art-header-tag-icon">
 +
<div class="t">FREiGEM 2009</div>
 +
</div>
 +
</div>
 +
<div class="art-BlockContent">
 +
<div class="art-BlockContent-body">
 +
<div>
 +
<p><b><img style="width: 138px; height: 40px;"
 +
alt="The Team"
 +
src="https://static.igem.org/mediawiki/2009/f/f6/Freiburg09_Team.gif" /></b></p>
 +
<p><b>The Team</b><br />
 +
This year our team consists of 18 undergraduate students and 4 advisors.<br />
 +
<a href="javascript:void(0)">Read more...</a></p>
 +
<p></p>
 +
<span style="text-decoration: underline;"><img
 +
style="width: 147px; height: 49px;" alt="bioss"
 +
src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_Bioss_portlet.jpg" /></span>
 +
<p><b>Bioss</b><br />
 +
We want to thank our main sponsors from the Center for Biological
 +
Signalling Studies, Bioss.<br />
 +
<a target="_blank" href="http://www.bioss.uni-freiburg.de">Read
 +
more...</a></p>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="art-Block">
 +
<div class="art-Block-tl"></div>
 +
<div class="art-Block-tr"></div>
 +
<div class="art-Block-bl"></div>
 +
<div class="art-Block-br"></div>
 +
<div class="art-Block-tc"></div>
 +
<div class="art-Block-bc"></div>
 +
<div class="art-Block-cl"></div>
 +
<div class="art-Block-cr"></div>
 +
<div class="art-Block-cc"></div>
 +
<div class="art-Block-body">
 +
<div class="art-BlockHeader">
 +
<div class="l"></div>
 +
<div class="r"></div>
 +
<div class="art-header-tag-icon">
 +
<div class="t">Visitor Locations</div>
 +
</div>
 +
</div>
 +
<div class="art-BlockContent">
 +
<div class="art-BlockContent-body">
 +
<div> &nbsp;<br />
 +
<a
 +
href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Freiburg_bioware"
 +
id="clustrMapsLink"><img
 +
src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Freiburg_bioware"
 +
style="border: 0px none ; width: 144px; height: 95px;"
 +
alt="Locations of visitors to this page"
 +
title="Locations of visitors to this page" id="clustrMapsImg"
 +
onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" />
 +
</a><br />
 +
<br />
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</body>
 +
</html>

Revision as of 14:26, 25 September 2009